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首页> 外文期刊>Advanced Materials >The Patterning and Alignment of Muscle Cells Using the Selective Adhesion of Poly(oligoethylene glycol methyl ether methacrylate)-based ABA Block Copolymers
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The Patterning and Alignment of Muscle Cells Using the Selective Adhesion of Poly(oligoethylene glycol methyl ether methacrylate)-based ABA Block Copolymers

机译：使用聚（低聚乙二醇甲基醚甲基丙烯酸甲酯）基ABA嵌段共聚物的选择性粘合对肌肉细胞进行构图和排列

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We have demonstrated how the selective adhesion/delamination behavior of the POEGMA-based polymers can be used to generate a functional 2D in-vitro model for muscle tissue. The procedure used to obtain this model is extremely simple, using only the spin-coating of a polymer solution onto a patterned substrate, followed by exposure of the substrate to cell culture medium and cell seeding (Fig. 3B). Compared with other cell-patterning methods previously reported in the literature that have also proved to be simple and efficient, our system is particularly suitable for cell patterning between electrodes. In addition to the preparation of the patterns of electrodes, our method involves only one step, the spin-coating of the copolymer film on the substrate. Afterwards, upon exposure to cell medium and cell seeding, delamination of the copolymer film from the hydrophilic regions occurs spontaneously. We have previously demonstrated that the selective adhe-sion/delamination behavior of the block copolymers 1 and 2 was not restricted to gold and glass substrates. In general, upon exposure to water the block copolymer films delaminate from various hydrophilic substrates (contact angle with water, θ = 10-50°), while being stable on hydrophobic substrates (θ = 70-90°). In principle, therefore, this method would allow for the generation of patterns of cells on a variety of substrates (with different flexibility), provided that they have been pre-patterned in hydrophilic and hydrophobic regions.
机译：我们已经证明了基于POEGMA的聚合物的选择性粘附/脱层行为如何可用于生成肌肉组织的功能性2D体外模型。用于获得此模型的过程非常简单，仅将聚合物溶液旋涂到有图案的基材上，然后将基材暴露于细胞培养基中并进行细胞接种（图3B）。与先前在文献中报道的其他已经证明简单有效的电池图案化方法相比，我们的系统特别适合于电极之间的电池图案化。除了准备电极图案外，我们的方法仅涉及一个步骤，即在基材上旋涂共聚物膜。此后，在暴露于细胞培养基和细胞接种后，共聚物膜从亲水区域自发地分层。先前我们已经证明，嵌段共聚物1和2的选择性粘合/脱层行为并不限于金和玻璃基板。通常，当暴露于水时，嵌段共聚物膜从各种亲水性基材上剥离（与水的接触角，θ＝ 10-50°），同时在疏水性基材上稳定（θ＝ 70-90°）。因此，原则上，只要已在亲水性和疏水性区域中预先构图了细胞，则该方法将允许在各种基材上（具有不同的柔韧性）生成细胞的图像。

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