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首页> 外文期刊>Aerobiologia >Siderophore production of African dust microorganisms over Trinidad and Tobago
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Siderophore production of African dust microorganisms over Trinidad and Tobago

机译:特立尼达和多巴哥上空非洲尘埃微生物的铁载体生产

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摘要

Iron (Fe) deposition from African dust has been implicated in a variety of environmental impacts on downwind terrestrial and marine ecosystems throughout the Caribbean. The most abundant form of Fe in African dust is Fe~Ⅲ, which is often not bioavailable. The objective of this study was to determine to what degree microorganisms isolated from African dust collected in Trinidad and Tobago are capable of producing siderophores that mobilize bioavailable Fe into the environment. Aerosol samples were collected for microbial analyses during African dust conditions in the source region (Mali) and downwind sites (Trinidad and Tobago). Microbial community fingerprints, obtained by means of terminal restriction fragment length polymorphism analysis, were compared among aerosol samples and possible Trinidadian sources of locally aerosolized microorganisms (sea water and soils). Ordination of the fingerprint data revealed similarities between aerosols from the source region and the aerosols and soils of downwind regions. Aerosol isolates from the downwind sites were screened for siderophore production using a modified chrome azurol-S (CAS) assay. Twenty-five percent of isolates tested that were sampled under non-dust conditions and 65% of African dust isolates produced at least one type of siderophore; among African dust isolates, all known classes of siderophores were produced. These data support African dust microorganism siderophore production as a viable mechanism by which Fe bioavail-ability may be increased in downwind locations, given appropriate conditions for microbial proliferation.
机译:非洲尘埃中的铁(Fe)沉积与整个加勒比地区顺风的陆地和海洋生态系统的各种环境影响有关。非洲粉尘中最丰富的铁形态是Fe〜Ⅲ,通常无法生物利用。这项研究的目的是确定从特立尼达和多巴哥收集的非洲粉尘中分离出的微生物能够在何种程度上产生铁载体,从而将可利用生物的铁迁移到环境中。在源地区(马里)和顺风地区(特立尼达和多巴哥)的非洲沙尘条件下,收集了气溶胶样品进行微生物分析。通过终端限制性片段长度多态性分析获得的微生物群落指纹,在气溶胶样品与可能的特立尼达本地气雾化微生物(海水和土壤)来源之间进行了比较。指纹数据的排序揭示了源区的气溶胶与顺风区的气溶胶和土壤之间的相似性。使用改良的铬天青-S(CAS)分析筛选了顺风部位的气溶胶分离物,以检测铁载体的产生。在非粉尘条件下取样的分离菌中有25%产生,至少65%的非洲粉尘菌产生了至少一种铁载体。在非洲的粉尘分离物中,生产了所有已知类别的铁载体。这些数据支持非洲粉尘微生物铁载体的生产,这是一种可行的机制,在适当的微生物繁殖条件下,可以在顺风地区提高铁的生物利用度。

著录项

  • 来源
    《Aerobiologia》 |2012年第3期|p.391-401|共11页
  • 作者单位

    Department of Crop and Soil Sciences, Cornell University, Ithaca, NY 14853, USA;

    Department of Life Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago;

    U.S. Geological Survey, 600 Fourth Street South, St.Petersburg, FL 33701, USA;

    Department of Life Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago;

    Department of Crop and Soil Sciences, Cornell University, Ithaca, NY 14853, USA,Department of Crop and Soil Sciences, Cornell University, 722 Bradfield Hall, Ithaca, NY 14853, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    african dust; bacteria; fungi; archaea; microbial siderophores; CAS; T-RFLP;

    机译:非洲尘埃菌;菌类;古细菌微生物铁载体;CAS;实时RFLP;

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