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Feasibility of using real-time optical methods for detecting the presence of viable bacteria aerosols at low concentrations in clean room environments

机译:使用实时光学方法检测洁净室环境中低浓度活菌气雾剂的可行性

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An experimental investigation was carried out to determine the agreement between two methods of viable bacteria aerosol detection. Various amounts of Bacillus globigii (BG) spores were aerosolized in 1-s bursts into a HEPA-filtered air stream and sampled simultaneously with a fluorescence aerosol particle sensor (FLAPS) and a slit to agar biological air sampler. The slit sampler incorporated 150-mm malt extract culture plates, which were incubated at 37°C for at least 12 h before culturable BG particles were counted in terms of colony-forming units (CFU). A relationship between CFU and optically detected viable bacteria particles was determined as culturable particle concentrations decreased. Through further analytical procedures, the FLAPS showed a limit of detection (LOD) of 4.2 bacterial particle/2.5 1 of sampled air or 1.7 x 103 m~(-3). This real-time bacteria aerosol monitor could be used to detect burst contamination events during a surgical procedure. The technology may be used for developing a dose-response relationship between bacterial particle exposure and infection, a tool potentially helpful in determining patient risk.
机译:为了确定两种可行的细菌气溶胶检测方法之间的一致性,进行了实验研究。将各种数量的球形芽孢杆菌(BG)孢子以1 s的脉冲气雾化为HEPA过滤的气流,并同时用荧光气溶胶粒子传感器(FLAPS)和琼脂生物空气采样器的缝隙进行采样。装有150毫米麦芽提取物培养板的狭缝取样器,将其在37°C下孵育至少12 h,然后根据菌落形成单位(CFU)对可培养的BG颗粒进行计数。随着可培养颗粒浓度的降低,确定了CFU和光学检测的活细菌颗粒之间的关系。通过进一步的分析程序,FLAPS的检出限(LOD)为4.2细菌颗粒/2.5 1采样空气或1.7 x 103 m〜(-3)。这种实时细菌气溶胶监测器可用于检测外科手术过程中的突发污染事件。该技术可用于建立细菌颗粒暴露与感染之间的剂量反应关系,这可能有助于确定患者风险。

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