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(1→ 3)-β-D-glucan in different background environments and seasons

机译:(1→3)-β-D-葡聚糖在不同背景环境和季节下

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This study was undertaken to investigate how the length of the extraction period influences the (1 →3)-β-D-glucan (β-glucan) yield and also to examine the background concentration of β-glucan as airborne β-glucan in outdoor environments in different seasons and as concentrations in airborne and floor dust in offices. To ensure compatibility between results obtained in different laboratories, it is important to use optimal and standardised methods to extract and quantify β-glucan. In this study, an extraction period of 60 min gave the highest β-glucan yield. The median concentration of β-glucan in 44 floor dust samples was 597μg g~(-1) dust. The median concentration of airborne β-glucan in offices was 5.1 ng m~(-3) in the summer and 2.3 ng m~(-3)in the winter, and the outdoor median concentration in towns was 6.8 ng m~(-3). The outdoor airborne con-centration of β-glucan was significantly lower in January, November and December than during the rest of year. In July, the median airborne concentra-tion of β-glucan was 14 times higher than in January. Furthermore, the airborne concentration of β-glucan was significantly higher in July than in March, April, May, September and October. In the summertime, we found that the indoor airborne concentration of β-glucan was lower than outdoor concentrations. This is in accordance with measurements of concentrations of airborne pollen and culturable fungal spores showing higher outdoor than indoor concentrations during the summer months.
机译:进行了这项研究,以研究提取时间的长短如何影响(1→3)-β-D-葡聚糖(β-葡聚糖)的产量,并研究室外使用的β-葡聚糖作为空气中的β-葡聚糖的背景浓度不同季节的环境,以及办公室中空气传播和地板灰尘的浓度。为了确保在不同实验室获得的结果之间的兼容性,重要的是使用最佳的标准化方法来提取和定量β-葡聚糖。在这项研究中,提取时间为60分钟可获得最高的β-葡聚糖产量。 44个地板粉尘样品中β-葡聚糖的中位浓度为597μgg〜(-1)粉尘。办公室中空气中的β-葡聚糖的中位数浓度在夏季为5.1 ng m〜(-3),在冬季为2.3 ng m〜(-3),城镇的室外中位数浓度为6.8 ng m〜(-3) )。 1月,11月和12月的室外空气中β-葡聚糖浓度明显低于一年中的其余时间。 7月,β-葡聚糖的空气中位数浓度比1月高14倍。此外,7月的空气中β-葡聚糖浓度显着高于3月,4月,5月,9月和10月。在夏季,我们发现室内空气中β-葡聚糖的浓度低于室外浓度。这是根据夏季夏季空气中花粉和可培养真菌孢子的浓度测量得出的,这些花粉的室外浓度高于室内浓度。

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