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Comparison of the biological content of air samples collected at ground level and at higher elevation

机译：在地面和更高海拔收集的空气样本的生物含量比较

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摘要

In this study, air samples were collected simultaneously at ground level and from a broadcast tower (open space) 238 m high using two BioSam-plers and two portable Biostage impactors. The sampling lasted up to 5 h, from morning to afternoon, and the experiments were independently conducted twice. The samples collected by the BioSamplers were analyzed for bacterial diversity and concentration, endotoxin and (1,3)-β-D-glucan content. Denaturing gradient gel electrophoresis (DGGE) fingerprinting, DNA sequencing, real-time quantitative polymerase chain reaction (qPCR), and Limulus amebocyte lysate (LAL) were used for the analyses. Air samples collected by the portable Biostage impactors were directly incubated at 25℃, and the culturable bacteria and fungi concentrations were manually counted. The results from the culture analysis indicated that the ground air samples had higher (statistically significant) concentrations of culturable bacteria and fungi than those taken at an elevation of 238 m. DGGE profiles of PCR products showed similar bacterial diversity in both ground-level and elevated air samples. Pseudomonas was identified by DNA sequencing as the predominant bacterial genus present. The SYBRrnqPCR tests revealed that the pooled air samples from both the ground and elevated levels had similar total bacterial concentrations, about 1,000 times their culturable ones. Although the endotoxin and (1,3)-β-D-glucan concentrations were observed to vary over the time, no statistically significant differences were observed in the pooled air samples from the ground and elevated levels over the course of the day. The results from this study elucidated the microbial differences in air at different elevations, and added to the body of evidence of atmospheric mixing of biological agents.
机译：在这项研究中，使用两个BioSam-plers和两个便携式Biostage撞击器同时从地面和238 m高的广播塔（露天空间）中收集了空气样本。从早上到下午，采样持续长达5个小时，并且实验独立进行了两次。分析了由BioSamplers收集的样品的细菌多样性和浓度，内毒素和（1,3）-β-D-葡聚糖含量。使用变性梯度凝胶电泳（DGGE）指纹图谱，DNA测序，实时定量聚合酶链反应（qPCR）和Li变形细胞溶解物（LAL）进行分析。将便携式Biostage撞击器收集的空气样品直接在25℃下孵育，并手动计算可培养细菌和真菌的浓度。培养分析的结果表明，地面空气样品的可培养细菌和真菌浓度比在238 m高处采集的要高。 PCR产物的DGGE谱在地面和高空空气样品中均显示出相似的细菌多样性。通过DNA测序鉴定假单胞菌为主要的细菌属。 SYBRrnqPCR测试表明，从地面和升高的水平收集的空气样本具有相似的总细菌浓度，约为可培养细菌浓度的1000倍。尽管观察到内毒素和（1,3）-β-D-葡聚糖的浓度随时间变化，但从地面收集的空气样本中并没有观察到统计学上的显着差异，并且在一天当中没有升高的水平。这项研究的结果阐明了不同海拔高度的空气中的微生物差异，并为生物制剂在大气中混合提供了证据。

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  • 来源
    《Aerobiologia》 |2010年第3期|P.233-244|共12页
  • 作者

    Kejun Li; Shuofei Dong; Yan Wu; Maosheng Yao;

  • 作者单位

    State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China;

    State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China;

    State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China;

    State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China;

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  • 正文语种 eng
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  • 关键词

    culturability; DGGE; DNA sequencing; qPCR; bacterial diversity; elevation; fungi;

    机译：可培养性DGGE;DNA测序;定量PCR;细菌多样性海拔;菌类;

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