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首页> 外文期刊>Aerobiologia >Airborne Poaceae pollen in Porto (Portugal) and allergenic profiles of several grass pollen types
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Airborne Poaceae pollen in Porto (Portugal) and allergenic profiles of several grass pollen types

机译：波尔图（葡萄牙）的空中禾本科花粉和几种草粉类型的致敏性

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摘要

This work aims to investigate the presence of airborne grass pollen and to identify antigenic and allergenic profiles from eight different grass species collected in the Porto region (Portugal). Poaceae airborne pollen, sampled using a Hirst-type volumetric trap during 2003-2007, was the second most abundant type, and high concentrations were found from April to August. Pollen proteins extracted from the eight grass species collected were separated by SDS-PAGE, being the allergenic profile investigated by immunoblotting using sera from atopic patients and maize profilin polyclonal antibody (ZmPRO3). Pollen extract profiles showed several bands ranging from 10 to 97 kDa. In immunoblotting studies, a low molecular weight protein (12-13 kDa) was recognized by profilin antibody. Also, in all pollen extracts except Zea mays, the IgE binding proteins of 12-13 kDa were detected in sera from the 25 patients with different sensitization profiles presenting high IgE values (>80 kU/l). This protein can be considered as a potential causal agent of the allergic respiratory diseases.
机译：这项工作旨在调查空中草粉的存在，并从在波尔图地区（葡萄牙）收集的八种不同草种中鉴定抗原和过敏原。 2003年至2007年期间使用赫斯特（Hirst）型体积阱捕获的禾本科空气传播花粉是第二富集型花粉，从4月至8月发现浓度很高。通过SDS-PAGE分离从八种草种中提取的花粉蛋白，该蛋白是通过使用特应性患者血清和玉米蛋白纤维蛋白多克隆抗体（ZmPRO3）进行免疫印迹研究的致敏性。花粉提取物谱显示出从10到97kDa的几个条带。在免疫印迹研究中，profilin抗体识别出低分子量蛋白质（12-13 kDa）。另外，在除玉米粉之外的所有花粉提取物中，在25名具有高IgE值（> 80 kU / l）的不同敏化谱的患者的血清中检测到12-13 kDa的IgE结合蛋白。该蛋白可以被认为是过敏性呼吸系统疾病的潜在病因。

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