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首页> 外文期刊>AIDS Research and Human Retroviruses >Study of the Genotypic Resistant Pattern in HIV-Infected Women and Children from Rural West Cameroon
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Study of the Genotypic Resistant Pattern in HIV-Infected Women and Children from Rural West Cameroon

机译：喀麦隆西部农村地区HIV感染的妇女和儿童的基因型耐药模式研究

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The distribution of antiretroviral (ARV) therapy resistance mutations among HIV-1 strains was evaluated in 39 postpartum women, one pregnant woman, and 12 HIV-positive babies (seven newborns and five children) living in rural west Cameroon. Thirty-five women and all newborns received a single dose of nevirapine (NVP) to prevent mother-to-child transmission of HIV-1; two women were ARV treated and three were ARV naive. Of the 52 viral strains examined, three were subtype B, 45 were classified into eight HIV-1 non-B subtypes, and four remained unclassifiable. Sequence analysis for genotypic drug resistance in the reverse transcriptase (RT) gene showed the presence of mutations associated with nonnucleoside RT inhibitor resistance in 20% of the samples from NVP-treated women and in 57% of those from treated newborns. Mutations associated with nucleoside RT inhibitors (M184V in one case and V118I in four cases) were found in five samples, despite being derived from ARV-naive patients. As expected, a greater frequency of mutations was found in the protease gene region. Of the sequences analyzed, 79% harbored five to seven specific mutations. The secondary mutations showed the typical protease inhibitor resistance-associated pattern for non-subtype B viruses, M36I being the predominant mutation (92.5% in women, 100% in babies). Other mutations frequently detected were K20I, L63P, H69K, and I13V. These findings confirm that resistance mutations can be detected in ARV-naive patients infected with non-B subtypes and emphasize an urgent need for studies assessing the impact of these mutations on the efficacy of subsequent ARV therapy and on the appearance of drug-resistant strains.
机译：在喀麦隆西部农村地区的39名产后妇女，1名孕妇和12名HIV阳性婴儿（七个新生儿和五个孩子）中评估了HIV-1菌株中抗逆转录病毒（ARV）治疗耐药性突变的分布。 35名妇女和所有新生儿均接受了单剂量奈韦拉平（NVP）预防母婴传播HIV-1；两名妇女接受抗逆转录病毒治疗，三名未接受抗逆转录病毒治疗。在检查的52个病毒株中，有3个属于B亚型，有45个被分类为8个HIV-1非B亚型，还有4个仍无法分类。逆转录酶（RT）基因的基因型药物抗性的序列分析显示，在20％来自NVP治疗的妇女和57％来自新生儿的样本中，存在与非核苷RT抑制剂抗性相关的突变。尽管来自未接受过ARV的患者，但仍在五个样本中发现了与核苷RT抑制剂相关的突变（1例为M184V，4例为V118I）。如所预期的，在蛋白酶基因区域中发现了更高的突变频率。在分析的序列中，有79％具有5至7个特定突变。二级突变显示出非B型病毒典型的蛋白酶抑制剂抗性相关模式，其中主要突变为M36I（女性为92.5％，婴儿为100％）。经常检测到的其他突变是K20I，L63P，H69K和I13V。这些发现证实，在感染了非B亚型的ARV初次感染的患者中可以检测到耐药性突变，并强调迫切需要进行研究，评估这些突变对随后的ARV治疗的功效以及耐药菌株的出现的影响。

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