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首页> 外文期刊>AIDS Research and Human Retroviruses >Fetal Allostimulation of Maternal Cells: A Potential Mechanism for Perinatal HIV Transmission following Obstetrical Hemorrhage
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Fetal Allostimulation of Maternal Cells: A Potential Mechanism for Perinatal HIV Transmission following Obstetrical Hemorrhage

机译：胎儿同种异体刺激的母体细胞：产科出血后围产期HIV传播的潜在机制。

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Our aim was to elucidate the mechanism by which HIV transmission is increased following obstetrical hemorrhage. We investigated whether fetal allostimulation of maternal cells, which could occur following fetal-to-maternal hemorrhage, increases proliferation, HIV replication, and cellular activation. Peripheral blood mononuclear cells (PBMCs) were collected from HIV-infected mothers and their infants to assess maternal–fetal allostimulation. Responses were compared to allostimulation with unrelated donors. Maternal and fetal cells were cocultured to assess allogeneic stimulation. Cell proliferation was measured by [3H]thymidine incorporation and cell activation was assessed via fluorochrome-labeled antibody staining and flow cytometric analysis. Virus production from HIV-infected maternal cells was quantitated by p24 enzyme-linked immunosorbent assay or by branched chain DNA assay. Allostimulation with fetal cells led to maternal cell proliferation. In women with unsuppressed viral loads, virus release was also enhanced following allostimulation of maternal cells with fetal cells. Fetal cells are capable of allogeneically stimulating maternal cells, with responses comparable to those seen following allostimulation with unrelated donors. Allostimulation of maternal cells by fetal cells results in statistically significant increases in proliferation and enhanced HIV replication, suggesting a possible physiological mechanism for mother-to-child transmission of HIV in women with obstetrical hemorrhage
机译：我们的目的是阐明产科出血后HIV传播增加的机制。我们调查了胎儿间的母体同种异体刺激是否可能发生，这种同种异动可能会在胎儿至母体出血后发生，从而增加增殖，HIV复制和细胞活化。从感染了HIV的母亲及其婴儿中收集外周血单个核细胞（PBMC），以评估母胎同种异体刺激。将反应与不相关供体的同种异体刺激进行比较。将母细胞和胎儿细胞共培养以评估同种异体刺激。通过[3 H]胸苷掺入测量细胞增殖，并通过荧光染料标记的抗体染色和流式细胞术分析评估细胞活化。通过p24酶联免疫吸附测定或通过支链DNA测定定量从HIV感染的母体细胞产生的病毒。胎儿细胞的同种异体刺激导致母体细胞增殖。在病毒载量不受抑制的女性中，母体细胞与胎儿细胞同体刺激后，病毒释放也得到增强。胎儿细胞能够同种异体刺激母体细胞，其反应可与不相关供体同种异体刺激后看到的反应相当。胎儿细胞对母体细胞的同种异体刺激导致统计学上显着的增殖增加和HIV复制增强，这提示了产科出血妇女中HIV母婴传播的可能生理机制

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