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首页> 外文期刊>American journal of applied sciences >Bioassay Guided Isolation of Antibacterial Compounds from Andrographis paniculata
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Bioassay Guided Isolation of Antibacterial Compounds from Andrographis paniculata

机译：生物测定法指导穿心莲中抗菌化合物的分离

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摘要

Problem statement: Chronic disease-causing bacteria of medical importance have developed resistance to antibiotics, hence, necessitating distinct and constant need for safe and efficient therapeutic agents. Plants are considered potent candidate for this aim. A way out of reducing antibiotic resistance and adverse effects on host is the employment of antibiotic resistance inhibitors of plant origin. Approach: About 5 kg pulverized Andrographis paniculata whole plant was macerated with MeOH at room temperature to get 305 g freeze dried MeOH extract. The bioautography of MeOH extract using Staphylococcus aureus and Proteus mirabilis as indicator organisms revealed the presence of two potent antibacterial compounds. MeOH extract was further fractionated and purified by silica gel column chromatography which led to the isolation of a diterpene lactone and an ent-labdane diterpene glycoside upon crystallization with absolute ethanol. Results: Two antibacterial compounds viz., 3-O-β-D-glucosyl-14-deoxyandrographolide and 14-deoxyandrographolide were successfully isolated and characterized. Their structures were exclusively elucidated through spectroscopic methods (UV, IR, ~1H- and ~(13)C NMR). Conclusion: A. paniculata possesses antibacterial activity and could be potential source of a new class of antibiotics that might be useful for infectious disease chemotherapy and control.
机译：问题陈述：具有医学重要性的引起慢性疾病的细菌已发展出对抗生素的抗药性，因此，对安全有效的治疗剂的独特需求和持续需求。为此目的，植物被认为是有力的候选者。降低抗生素抗性和对宿主不利影响的一种方法是采用植物来源的抗生素抗性抑制剂。方法：在室温下将约5kg粉碎的穿心莲整个植物用MeOH浸软，得到305g冷冻干燥的MeOH提取物。使用金黄色葡萄球菌和奇异变形杆菌作为指示生物的MeOH提取物的生物自显影显示了两种有效的抗菌化合物的存在。将MeOH提取物进一步分馏并通过硅胶柱色谱法纯化，这导致在用无水乙醇结晶后分离出二萜内酯和对马丹烷二萜糖苷。结果：成功分离并鉴定了两种抗菌化合物，即3-O-β-D-葡萄糖基-14-脱氧穿心莲内酯和14-脱氧穿心莲内酯。通过光谱方法（UV，IR，〜1H-和〜（13）C NMR）专门阐明了它们的结构。结论：沙门氏菌具有抗菌活性，可能是新型抗生素的潜在来源，可能对传染病的化学疗法和控制有用。

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  • 来源
    《American journal of applied sciences》 |2011年第6期|p.525-534|共10页
  • 作者

    Abubakar Sule; Qamar Uddin Ahmed; Othman Abd. Samah; Muhammad Nor Omar; Norazian M. Hassan; Laina Zarisa M. Kamal; M. Ambar Yarmo;

  • 作者单位

    Department of Biomedical Sciences, Kulliyyah of Science, International Islamic University Malaysia, Kuantan, Pahang DM,;

    Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, 25200-Kuantan, Pahang DM, Malaysia;

    Department of Biomedical Sciences, Kulliyyah of Science, International Islamic University Malaysia, Kuantan, Pahang DM,;

    Department of Biomedical Sciences, Kulliyyah of Science, International Islamic University Malaysia, Kuantan, Pahang DM,;

    Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang DM,;

    Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang DM,;

    School of Chemical Sciences and Food Technology, Kulliyyah of Science and Technology, University Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Malaysia;

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  • 正文语种 eng
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  • 关键词

    andrographis paniculata; bioassay-guided isolation; antibacterial compounds;

    机译：穿心莲生物测定指导的分离;抗菌化合物;

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