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Assessing Soil Biological Properties of Natural and Planted Forests in the Malaysian Tropical Lowland Dipterocarp Forest

机译:马来西亚热带低地龙脑香林的天然和人工林土壤生物学特性评估

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Problem statement: A study was conducted to evaluate and compare the soil biological properties of a natural forest and an 18-year-old stand of Shorea leprosula in Chikus Forest Reserve, Perak, Malaysia. Approach: Soils were sampled at depths of 0-15 cm (topsoil) and 15-30 cm (subsoil) in six subplots (20x20 m) of natural forest (Cl) and of a planted S. leprosula (C2) plot. Fresh composite soil samples were kept in UV-sterilized polyethylene bags prior to analysis in the laboratory. The microbial population count was determined using a spread-plate count technique. The microbial enzymatic activity was elucidated using a Fluorescein Diacetate (FDA) hydrolysis assay; microbial biomass was extracted using a rapid chloroform fumigation extraction method. The Microbial Biomass C (MBC) was determined by wet dichromate oxidation; Kjeldahl digestion and a distillation method were used for evaluation of Microbial Biomass N (MBN). Results: Results indicate that only the microbial biomass N and the population count in the soil at the 0-15 cm depth were found to be higher in Cl compared to C2. The higher microbial population count in the soil at the 0-15 cm depth of C1 compared to C2 was enhanced by the large amount of organic matter that serves as a suitable medium for soil microbial growth. The higher MBN in the Cl soil was also influenced by the high content of organic material available that encourages activities of decomposing bacteria to take place. Similarities in the soil biological properties of the plots with regard to enzymatic activity and microbial biomass Care believed to be influenced by the same topographic gradient. The higher MBC/MBN ratios found in soils of C2 compared to Cl were due to the low availability of N compared to C, might result from N utilization by soil microbes for organic material decomposition. Conclusion: There are similarities in microbial enzymatic activity and biomass C, but not in microbial population counts and biomass N, between a natural forest and an 18-year-old stand of S. leprosula in Chikus Forest Reserve, Perak, Malaysia.
机译:问题陈述:进行了一项研究,以评估和比较马来西亚霹雳州奇库斯森林保护区的天然林和18岁的麻风树的土壤生物学特性。方法:在天然林(C1)和种植的麻风链霉菌(C2)地块的六个子图(20x20 m)中,在0-15 cm(表土)和15-30 cm(底土)的深度取样土壤。在实验室进行分析之前,将新鲜的复合土壤样品保存在经过紫外线消毒的聚乙烯袋中。使用扩展板计数技术确定微生物种群计数。使用二乙酸荧光素(FDA)水解测定法阐明了微生物的酶活性。使用快速氯仿熏蒸提取方法提取微生物生物量。微生物生物质C(MBC)通过湿重铬酸盐氧化测定;凯氏消化法和蒸馏法用于评估微生物生物量N(MBN)。结果:结果表明,与C2相比,C1中只有0-15 cm深度的微生物量和土壤微生物数量更高。与C2相比,C1在0-15厘米深度处土壤中的微生物数量更高,这是由于大量有机物可作为土壤微生物生长的合适介质。 Cl土壤中较高的MBN还受到可利用的高有机物质含量的影响,这些物质可促进细菌分解活动的发生。在酶活性和微生物生物量方面,样地的土壤生物学特性相似。据信受相同地形梯度影响。与C相比,C2土壤中的MBC / MBN比值更高,这是由于N与C相比氮的利用率低,这可能是由于土壤微生物对氮进行有机物质分解而导致的。结论:马来西亚霹雳州奇库斯森林保护区的天然林和18岁的麻风链球菌在微生物酶活性和生物量碳方面相似,但在微生物种群数量和生物量氮方面没有相似之处。

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