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A chemotaxonomic analysis of cannabinoid variation in Cannabis (Cannabaceae)

机译：大麻中大麻素变化的化学分类学分析

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摘要

Cannabinoids are important chemotaxonomic markers unique to Cannabis. Previous studies show that a plant's dry-weight ratio of 9-tetrahydrocannabinol (THC) to cannabidiol (CBD) can be assigned to one of three chemotypes and that alleles BD and BT encode alloenzymes that catalyze the conversion of cannabigerol to CBD and THC, respectively. In the present study, the frequencies of BD and BT in sample populations of 157 Cannabis accessions were determined from CBD and THC banding patterns, visualized by starch gel electrophoresis. Gas chromatography was used to quantify cannabinoid levels in 96 of the same accessions. The data were interpreted with respect to previous analyses of genetic and morphological variation in the same germplasm collection. Two biotypes (infraspecific taxa of unassigned rank) of C. sativa and four biotypes of C. indica were recognized. Mean THC levels and the frequency of BT were significantly higher in C. indica than C. sativa. The proportion of high THC/CBD chemotype plants in most accessions assigned to C. sativa was 25%. Plants with relatively high levels of tetrahydrocannabivarin (THCV) and/or cannabidivarin (CBDV) were common only in C. indica. This study supports a two-species concept of Cannabis.
机译：大麻素是 大麻特有的重要化学分类标记。先前的研究表明，植物的 9 -四氢大麻酚（THC）与大麻二酚（CBD）的干重比 可以被指定为以下三个之一化学型，等位基因B D 和 B T 编码的同工酶催化大麻脂 转化为CBD和THC，分别。在本研究中，确定了157个大麻登录名 的样本群体中B D 和B T 的频率 由CBD和THC条带图谱显示，通过淀粉凝胶电泳显示 。气相色谱法用于 定量分析96种相同成分中的大麻素水平。 数据是针对先前对同一种质集合中的基因 和形态变异进行的分析而解释的。 两种生物类型（未分配等级的亚种下分类群）认识到了四种C. indica C. sativa 。 C稻的平均THC水平 和B T 的频率明显高于紫花苜蓿。高THC / CBD化学型植物 在分配给苜蓿中华根瘤菌的大多数种中的比例为25％。具有 相对较高水平的四氢大麻酚（THCV）和/或 cannabidivarin（CBDV）的植物仅在印度C中普遍存在。这项研究 支持大麻的两种物种概念。

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