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首页> 外文期刊>Animal Genetics >Construction of integrated genetic linkage maps of the tiger shrimp (Penaeus monodon) using microsatellite and AFLP markers
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Construction of integrated genetic linkage maps of the tiger shrimp (Penaeus monodon) using microsatellite and AFLP markers

机译：利用微卫星和AFLP标记构建老虎虾（斑节对虾）综合遗传连锁图谱

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摘要

SummaryThe linkage maps of male and female tiger shrimp (P. monodon) were constructed based on 256 microsatellite and 85 amplified fragment length polymorphism (AFLP) markers. Microsatellite markers obtained from clone sequences of partial genomic libraries, tandem repeat sequences from databases and previous publications and fosmid end sequences were employed. Of 670 microsatellite and 158 AFLP markers tested for polymorphism, 341 (256 microsatellite and 85 AFLP markers) were used for genotyping with three F1 mapping panels, each comprising two parents and more than 100 progeny. Chi-square goodness-of-fit test (χ2) revealed that only 19 microsatellite and 28 AFLP markers showed a highly significant segregation distortion (P < 0.005). Linkage analysis with a LOD score of 4.5 revealed 43 and 46 linkage groups in male and female linkage maps respectively. The male map consisted of 176 microsatellite and 49 AFLP markers spaced every ∼11.2 cM, with an observed genome length of 2033.4 cM. The female map consisted of 171 microsatellite and 36 AFLP markers spaced every ∼13.8 cM, with an observed genome length of 2182 cM. Both maps shared 136 microsatellite markers, and the alignment between them indicated 38 homologous pairs of linkage groups including the linkage group representing the sex chromosome. The karyotype of P. monodon is also presented. The tentative assignment of the 44 pairs of P. monodon haploid chromosomes showed the composition of forty metacentric, one submetacentric and three acrocentric chromosomes. Our maps provided a solid foundation for gene and QTL mapping in the tiger shrimp.
机译：摘要基于256个微卫星和85个扩增片段长度多态性（AFLP）标记构建了雄性和雌性虎虾（斑节对虾）的连锁图谱。使用了从部分基因组文库的克隆序列，数据库的串联重复序列和以前的出版物以及fosmid末端序列获得的微卫星标记。在测试的670个微卫星和158个AFLP标记的多态性中，有341个（256个微卫星和85个AFLP标记）用于三个F 1 定位图谱的基因分型，每个定位图包括两个亲本和100多个后代。卡方拟合优度检验（χ 2 ）显示，只有19个微卫星和28个AFLP标记显示出高度显着的偏析变形（P <0.005）。 LOD得分为4.5的连锁分析显示，男性和女性连锁图中分别有43和46个连锁组。雄性图由176个微卫星和49个AFLP标记组成，每11.2 cM间隔，观察到的基因组长度为2033.4 cM。雌性图由171个微卫星和36个AFLP标记组成，每13.8 cM间隔，观察到的基因组长度为2182 cM。两张图共有136个微卫星标记，它们之间的比对表明38对同源的连锁基团，包括代表性染色体的连锁基团。还介绍了斑节对虾的核型。初步确定了44对斑节对虾单倍体染色体的组成，显示了40个亚中心，一个亚中心和三个近中心染色体的组成。我们的地图为虎虾的基因和QTL定位奠定了坚实的基础。

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  • 来源
    《Animal Genetics》 |2010年第4期|p.365-376|共12页
  • 作者

    E.-M. You; K.-F. Liu; S.-W. Huang; M. Chen; M. L. Groumellec; S.-J. Fann; H.-T. Yu;

  • 作者单位

    Institute of Zoology and Department of Life Science, National Taiwan University, Taipei, 10617, Taiwan, Republic of China;

    Tungkang Biotechnology Research Center, Fisheries Research Institute, C. O. A., Pingtung, 92845, Taiwan, Republic of China;

    Institute of Zoology and Department of Life Science, National Taiwan University, Taipei, 10617, Taiwan, Republic of China;

    Center for Medical Genetics, Department of Genomic Medicine, Changhua Christian Hospital, Changhua city, Changhua County, 500, Taiwan, Republic of China;

    Aquaculture de la Mahajamba, Unima, Mahajanga 401, Madagascar;

    Institute of Biomedical Science, Academia Sinica, Taipei, 115, Taiwan, Republic of China;

    Institute of Zoology and Department of Life Science, National Taiwan University, Taipei, 10617, Taiwan, Republic of China;

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  • 关键词

    amplified fragment length polymorphism; Fsub1/sub mapping panel; karyotype; linkage maps; microsatellite; Penaeus monodon;

    机译：扩增片段长度多态性;F sub 1 / sub定位图;核型;连锁图;微卫星;对虾;

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