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首页> 外文期刊>Animal Science Journal >Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm
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Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm

机译：线粒体DNA在由沼泽水牛成纤维细胞和牛卵质重建的跨基因克隆胚胎中的恒定传递

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摘要

Although interspecies/intergeneric somatic cell nuclear transfer (iSCNT) has been proposed as a tool to produce offspring of endangered species, conflict between donor nucleus and recipient cytoplasm in iSCNT embryos has been identified as an impediment to implementation for agricultural production. To investigate the nuclear–mitochondrial interactions on the developmental potential of iSCNT embryos, we analyzed the mtDNA copy numbers in iSCNT embryos reconstructed with water buffalo (swamp type) fibroblasts and bovine enucleated oocytes (buffalo iSCNT). As controls, SCNT embryos were derived from bovine fibroblasts (bovine SCNT). Buffalo iSCNT and bovine SCNT embryos showed similar rates of cleavage and development to the 8-cell stage (P?>?0.05). However, buffalo iSCNT embryos did not develop beyond the 16-cell stage. Both bovine and buffalo mtDNA content in buffalo iSCNT embryos was stable throughout the nuclear transfer process, and arrested at the 8- to 16-cell stage (P?>?0.05). In bovine SCNT embryos that developed to the blastocyst stage, mtDNA copy number was increased (P?
机译：尽管已经提出种间/种间体细胞核转移（iSCNT）作为生产濒危物种后代的工具，但iSCNT胚胎中供体核与受体细胞质之间的冲突已被确定为实现农业生产的障碍。为了研究核线粒体相互作用对iSCNT胚胎发育潜力的影响，我们分析了水牛（沼泽型）成纤维细胞和牛去核卵母细胞（buffalo iSCNT）重建的iSCNT胚胎中的mtDNA拷贝数。作为对照，SCNT胚胎来自牛成纤维细胞（牛SCNT）。布法罗iSCNT和牛SCNT胚胎的分裂和发育速率与8细胞期相似（P≥0.05）。但是，水牛iSCNT胚胎没有发育超过16细胞阶段。在整个核移植过程中，水牛iSCNT胚胎中的牛和水牛mtDNA含量均稳定，并停留在8至16个细胞的阶段（P≥0.05）。在发育到胚泡期的牛SCNT胚胎中，mtDNA拷贝数增加（P≤0.05）。总之，水牛iSCNT胚胎的供体细胞和受体细胞质体mtDNA均已鉴定，并通过iSCNT过程得以维持直至8-16个细胞阶段。此外，每个胚胎的mtDNA拷贝数是研究核线粒体相互作用的有用监测器。

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