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首页> 外文期刊>Annals of the New York Academy of Sciences >Role of NADPH Oxidase and Calcium in Cerulein-Induced Apoptosis Involvement of Apoptosis-Inducing Factor
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Role of NADPH Oxidase and Calcium in Cerulein-Induced Apoptosis Involvement of Apoptosis-Inducing Factor

机译：NADPH氧化酶和钙在Cerulein诱导的细胞凋亡过程中的作用

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Apoptosis linked to oxidative stress has been implicated in pancreatitis. NADPH oxidase has been considered as a major source of reactive oxygen species (ROS) during inflammation and apoptosis in pancreatic acinar cells. Recently we demonstrated that NADPH oxidase sub-units Nox1, p27~(phox), p47~(phox), and p67~(phox) are constitutively expressed in pancreatic acinar cells and may contribute to apoptosis in pancreatic acinar AR42J cells stimulated with cerulein. The present study aims to investigate the apoptotic mechanism of pancreatic acinar cells stimulated with cerulein by determining whether cerulein induces apoptosis-inducing factor (AIF) expression and whether cerulein-induced expression of AIF is inhibited by transfection with antisense oligonucleotide (AS ODN) of p47~(phox) or p67~(phox) or treatment with a Ca~(2+) chelator BAPTA-AM. As a result, cerulein induced the expression of apoptotic gene AIF. Transfection with AS ODN of p47~(phox) or p67~(phox) or treatment with BAPTA-AM inhibited cerulein-induced AIF expression in pancreatic acinar AR42J cells. These results demonstrate that NADPH oxidase and calcium have a role in cerulein-induced apoptosis in pancreatic acinar AR42J cells by inducing the expression of AIF. In conclusion, the increase in intracel-lular Ca~(2+) and NADPH oxidase activity may be the upstream event of apoptotic gene (AIF) expression, which contributes to cerulein-induced apoptosis in pancreatic acinar AR42 cells.
机译：与氧化应激有关的细胞凋亡与胰腺炎有关。 NADPH氧化酶被认为是胰腺腺泡细胞炎症和凋亡过程中活性氧（ROS）的主要来源。最近，我们证明了NADPH氧化酶亚基Nox1，p27〜（phox），p47〜（phox）和p67〜（phox）在胰腺腺泡细胞中组成性表达，并可能促进了由轻蓝素刺激的胰腺腺泡AR42J细胞的凋亡。本研究旨在通过测定铜绿蛋白是否诱导凋亡诱导因子（AIF）表达以及铜绿蛋白诱导的AIF表达是否被p47反义寡核苷酸（AS ODN）转染抑制来研究铜蓝蛋白刺激的胰腺腺泡细胞的凋亡机制。 〜（phox）或p67〜（phox）或用Ca〜（2+）螯合剂BAPTA-AM处理。结果，铜蓝蛋白诱导了凋亡基因AIF的表达。 p47〜（phox）或p67〜（phox）的AS ODN转染或BAPTA-AM处理抑制了胰蛋白酶诱导的胰腺腺癌AR42J细胞中AIF表达。这些结果表明，NADPH氧化酶和钙通过诱导AIF的表达而在小分子蛋白酶诱导的胰腺腺泡AR42J细胞凋亡中起作用。总之，细胞内Ca〜（2+）和NADPH氧化酶活性的增加可能是细胞凋亡基因（AIF）表达的上游事件，这可能是由青霉素诱导的胰腺腺泡AR42细胞凋亡。

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