Identification of Antigenic Proteins of a Theileria Species Pathogenic for Small Ruminants in China Recognized by Antisera of Infected Animals

JOANA MIRANDA; BARBARA STUMME; DOREEN BEYER; HELDER CRUZ; ABEL GONZALEZ OLIVA; MOHAMMED BAKHEIT; DANIEL WICKLEIN; HONG YIN; JIANXUN LOU; JABBAR S. AHMED; ULRIKE SEITZER

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Identification of Antigenic Proteins of a Theileria Species Pathogenic for Small Ruminants in China Recognized by Antisera of Infected Animals

机译：感染动物抗血清识别的中国小反刍动物致病菌泰勒菌种的抗原蛋白鉴定

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The antigenic proteins of the piroplasm stage of Theileria species (China), the causative agent of theilerosis of small ruminants in China, were analyzed by Western blot, revealing several specific immunoreactive proteins of different predicted molecular weights. Furthermore, sera from Theileria species (China)-infected animals were probed for reactivity with the TaSP protein of T. annulata, for which a homologue has been described in Theileria species (China). Affinity chromatography demonstrated the presence of TaSP-reactive antibodies, and the majority of the sera showed reactivity with this protein both in Western blots and in ELISA. The identified parasite antigens and TaSP will be assessed for their suitability for developing diagnostic methods as well as evaluated for their capacity to stimulated host immune competent cells.

机译：通过蛋白质印迹法分析了中国小反刍动物热病的病原体Theileria种（中国）的质体阶段的抗原蛋白，揭示了几种具有不同预测分子量的特异性免疫反应蛋白。此外，还检测了来自泰勒虫属（中国）感染动物的血清与环孢菌（Ta。annulata）的TaSP蛋白的反应性，在泰勒虫属（中国）中已描述了其同系物。亲和色谱法表明存在TaSP反应性抗体，并且大多数血清在Western印迹和ELISA中均显示与该蛋白的反应性。将对鉴定出的寄生虫抗原和TaSP进行开发诊断方法的适用性进行评估，并评估其刺激宿主免疫感受态细胞的能力。

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来源
《Annals of the New York Academy of Sciences》 |2004年第2004期|p.161-164|共4页
作者
JOANA MIRANDA; BARBARA STUMME; DOREEN BEYER; HELDER CRUZ; ABEL GONZALEZ OLIVA; MOHAMMED BAKHEIT; DANIEL WICKLEIN; HONG YIN; JIANXUN LOU; JABBAR S. AHMED; ULRIKE SEITZER;
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Instituto de Biologia Experimental e Tecnologica, Universidade Nova de Lisboa, Oeiras, Portugal;

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正文语种 eng
中图分类自然科学总论;
关键词
theileria species (China); piroplasm stage; TaSP cross-reactivity;

机译：泰勒菌种（中国）;质浆期;TaSP交叉反应;

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专利

1. Identification and Characterization of Merozoite Antigens of a Theileria Species Highly Pathogenic for Small Ruminants in China [J] . JOANA MIRANDA, ELISABETE NASCIMENTO, HELDER CRUZ, Annals of the New York Academy of Sciences . 2006,第Oct期

机译：中国小反刍动物致病菌泰勒菌种的裂殖子抗原的鉴定与鉴定
2. Ribosomal small-subunit RNA gene-sequence analysis of Theileria lestoquardi and a Theileria species highly pathogenic for small ruminants in China. [J] . Schnittger L, Yin H, Jianxun L, Parasitology Research . 2000,第5期

机译：中国小反刍动物致病菌泰勒菌和泰勒菌种的核糖体小亚基RNA基因序列分析。
3. Characterization of a polymorphic gene of T. lestoquardi and of a recently identified Theileria species pathogenic for small ruminants in China. [J] . Schnittger L, Yin H, Luo J, Parasitology Research . 2002,第6期

机译：T. lestoquardi多态性基因和最近鉴定出的中国小反刍动物致病菌泰勒菌种的鉴定。
4. Identification of Animal Species by the MALDI-MS of Collagen in Animal Glues of Chinese Ink Sticks [C] . Kazuki Kawahara, Yuzo Yamazaki, Atsuko Ogami, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：中国墨棒胶原胶中MALDI-MS鉴定动物物种
5. Development of a cloth-based hybridization array system for the detection and identification of ruminant species in animal feed. [D] . Armour, Jennifer. 2005

机译：基于布料的杂交阵列系统的开发，用于检测和识别动物饲料中的反刍动物。
6. Borrelia burgdorferi B31 Erp Proteins That Are Dominant Immunoblot Antigens of Animals Infected with Isolate B31 Are Recognized by Only a Subset of Human Lyme Disease Patient Sera [O] . Jennifer C. Miller, Nazira El-Hage, Kelly Babb, 2000

机译：仅被人类莱姆病患者血清亚型识别的伯瑞氏疏螺旋体B31 Erp蛋白是被分离株B31感染的动物的主要免疫印迹抗原。
7. Borrelia burgdorferi B31 Erp Proteins That Are Dominant Immunoblot Antigens of Animals Infected with Isolate B31 Are Recognized by Only a Subset of Human Lyme Disease Patient Sera [O] . Jennifer C. Miller, Nazira El-Hage, Kelly Babb, 2000

机译：Borrelia Burgdorferi B31 ERP蛋白质，其具有分离物B31感染的动物的占主导地免疫印迹抗原，仅通过人莱姆病患者血清的子集来识别

Identification of Antigenic Proteins of a Theileria Species Pathogenic for Small Ruminants in China Recognized by Antisera of Infected Animals

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