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首页> 外文期刊>Annals of the New York Academy of Sciences >Sympathetic innervation of the interscapular brown adipose tissue in mouse
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Sympathetic innervation of the interscapular brown adipose tissue in mouse

机译：小鼠肩s间棕色脂肪组织的交感神经

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The recent discovery of significant brown fat depots in adult humans has revived discussion of exploiting brown fat thermogenesis in the control of energy balance and body weight. The sympathetic nervous system (SNS) has a key role in the activation of brown fat and functional mapping of its components will be crucial for the development of specific neuromodulation techniques. The mouse is an important species used for molecular genetic modulations, but its small size is not ideal for anatomical dissections, thus brown fat innervation studies are mostly available in larger rodents such as rats and hamsters. Here, we use pseudorabies virus retrograde tracing, whole tissue clearing, and confocal/light sheet microscopy to show the location of pre- and postganglionic neurons selectively innervating the interscapular brown adipose tissue (iBAT) in the mouse. Using iDISCO whole tissue clearing, we identified iBAT projecting postganglionic neurons in the caudal parts of the ipsilateral fused stellate/T1, as well as the T2-T5 sympathetic chain ganglia and preganglionic neurons between levels T2 and T6 of the ipsilateral spinal cord. The methodology enabled high-resolution imaging and 3D rendering of the specific SNS innervation of iBAT and will be helpful to discern peripheral nervous system innervation of other organs and tissues.
机译：最近在成年人中发现了重要的棕色脂肪储藏库，使人们重新讨论了利用棕色脂肪生热来控制能量平衡和体重的问题。交感神经系统（SNS）在棕色脂肪的活化中起关键作用，其组成部分的功能定位对于特定神经调节技术的发展至关重要。小鼠是用于分子遗传调控的重要物种，但其小巧的体积并不适合解剖解剖，因此棕色脂肪神经支配研究主要适用于大型啮齿动物，例如大鼠和仓鼠。在这里，我们使用伪狂犬病病毒逆行追踪，整个组织清除和共聚焦/光片显微镜检查来显示神经节前和神经节后神经元的位置，这些神经元选择性地支配了小鼠肩brown间棕色脂肪组织（iBAT）。使用iDISCO整个组织清除，我们在同侧融合星状体/ T1的尾部以及同侧脊髓的T2和T6之间的T2-T5交感神经节神经节和前神经节神经元中识别了iBAT投射的节后神经元。该方法能够对iBAT的特定SNS神经支配进行高分辨率成像和3D渲染，并将有助于辨别其他器官和组织的周围神经系统神经支配。

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