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首页> 外文期刊>Antonie van Leeuwenhoek >Jeotgalibacillus soli sp. nov., isolated from non-saline forest soil, and emended description of the genus Jeotgalibacillus
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Jeotgalibacillus soli sp. nov., isolated from non-saline forest soil, and emended description of the genus Jeotgalibacillus

机译:索氏芽孢杆菌十一月,从非盐碱森林土壤中分离出来,并对Jeotgalibacillus属的描述进行了改进

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摘要

A Gram-stain-positive, endospore-forming, motile, catalase- and oxidase-positive, aerobic, rod-shaped bacterium, designated strain JSM 081008T, was isolated from non-saline forest soil in China. Strain JSM 081008T was able to grow with 0–20% (w/v) NaCl, at pH 6.0–10.5 and at 10–45°C; optimum growth was observed with 2–5% (w/v) NaCl, at pH 7.0–8.0 and at 30–35°C. The peptidoglycan type was A1α linked directly through l-Lys. The major cellular fatty acids (>10% of the total) were anteiso-C15:0, iso-C15:0, anteiso-C17:0 and C16:0. The predominant respiratory quinone was menaquinone 7 and the genomic DNA G + C content of the strain was 42.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 081008T should be assigned to the genus Jeotgalibacillus and was related most closely to the type strains of Jeotgalibacillus alimentarius (sequence similarity 99.4%) and Jeotgalibacillus salarius (97.0%), followed by Jeotgalibacillus campisalis (95.4%) and Jeotgalibacillus marinus (95.2%). The combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the view that strain JSM 081008T represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus soli sp. nov. is proposed. The type strain is JSM 081008T (=DSM 22174T = KCTC 13528T). An emended description of the genus Jeotgalibacillus is also presented.
机译:从中国非盐碱森林土壤中分离出革兰氏染色阳性,内生孢子,运动型,过氧化氢酶和氧化酶阳性的好氧杆状细菌,命名为JSM 081008 T 。 。 JSM 081008 T 菌株能够在0–20%(w / v)NaCl,pH 6.0–10.5和10–45°C的条件下生长。在pH 7.0-8.0和30-35°C下,使用2–5%(w / v)NaCl可以观察到最佳生长。肽聚糖类型为A1α,直接通过l-Lys连接。主要的细胞脂肪酸(占总数的> 10%)为前异-C15:0,异-C15:0,前异-C17:0和C16:0。主要的呼吸醌为甲萘醌7,该菌株的基因组DNA G + C含量为42.6 mol%。根据16S rRNA基因序列的系统进化分析表明,应将JSM 081008 T 菌株归入Jeotgalibacillus属,并且与alimentarius Jeotgalibacillus(序列相似性99.4%)和Salotgalibacillus salarius(序列相似性)的菌株最相关。 97.0%),其次是喜多加弯曲杆菌(95.4%)和海藻加多弯曲杆菌(95.2%)。系统发育分析,DNA-DNA相关性值,表型特征和化学分类数据的结合,支持了以下观点:菌株JSM 081008 T 代表Jeotgalibacillus属的一种新种,其名称为Jeotgalibacillus soli sp。十一月被提议。类型应变为JSM 081008 T (= DSM 22174 T = KCTC 13528 T )。还提出了对变形杆菌属的改良描述。

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