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首页> 外文期刊>Apoptosis >Rapid cold-hardening blocks cold-induced apoptosis by inhibiting the activation of pro-caspases in the flesh fly Sarcophaga crassipalpis
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Rapid cold-hardening blocks cold-induced apoptosis by inhibiting the activation of pro-caspases in the flesh fly Sarcophaga crassipalpis

机译：快速冷硬化通过抑制果蝇Sarcophaga crassipalpis中前胱天蛋白酶的激活来阻断冷诱导的细胞凋亡

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摘要

Apoptosis plays important roles in the selective elimination of sub-lethally damaged cells due to various environmental stresses. The rapid cold-hardening (RCH) response protects insects from the otherwise lethal consequences of injury due to cold-shock. We recently demonstrated that cold shock induces apoptotic cell death in insects and that RCH functions to specifically block cold-shock-induced apoptosis. In the present study we used isolated fat body, midgut, muscle, and Malpighian tubules from adult flesh flies Sarcophaga crassipalpis to test the following hypotheses: (1) cold-induced apoptosis varies among different tissues and (2) RCH blocks the apoptotic pathway by preventing the activation of pro-caspases. Cold-shock induced substantial amounts of apoptotic cell death that matched with tissue damage as determined using vital dyes. RCH treatment significantly reduced apoptotic cell death in all tested tissues. Caspase-3 (executioner) activity was 2–3 times higher in the cold- and heat-shocked groups than in control and RCH groups. Likewise, the activity of caspase-9 (initiator) showed a similar trend as for caspase-3 in all tissues but midgut. In addition, cold-shock and heat-shock treatments also increased caspase-2 activity 2–3 folds in both soluble and membrane fractions of fat body and muscle extracts compared to controls.
机译：凋亡在选择性消除由于各种环境压力而导致的亚致死细胞中发挥重要作用。快速冷硬化（RCH）响应可保护昆虫免受冷冲击造成的致命伤害。我们最近证明冷休克诱导昆虫凋亡细胞死亡，而RCH的功能是特异性阻断冷休克诱导的细胞凋亡。在本研究中，我们使用了来自成年果蝇Sarcophaga crassipalpis的分离的脂肪小体，中肠，肌肉和Malpighian小管，以检验以下假设：（1）冷诱导的细胞凋亡在不同组织之间不同，（2）RCH通过以下途径阻断细胞凋亡途径防止前胱天蛋白酶的激活。冷休克诱导大量凋亡细胞死亡，与使用重要染料确定的组织损伤相匹配。 RCH治疗显着降低了所有测试组织中的凋亡细胞死亡。冷激和热激组的Caspase-3（执行者）活性是对照组和RCH组的2-3倍。同样，caspase-9（引发剂）的活性在中肠以外的所有组织中均表现出与caspase-3相似的趋势。此外，与对照组相比，冷冲击和热冲击处理还使脂肪体和肌肉提取物的可溶性和膜部分的caspase-2活性增加了2-3倍。

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