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首页> 外文期刊>Apoptosis >Pro-apoptotic cell death genes, hid and reaper, from the tephritid pest species, Anastrepha suspensa
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Pro-apoptotic cell death genes, hid and reaper, from the tephritid pest species, Anastrepha suspensa

机译：死于特霉病虫种Anastrepha suspensa的促凋亡细胞死亡基因hid和reaper

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Pro-apoptotic proteins from the reaper, hid, grim (RHG) family are primary regulators of programmed cell death in Drosophila due to their antagonistic effect on inhibitor of apoptosis (IAP) proteins, thereby releasing IAP-inhibition of caspases that effect apoptosis. Using a degenerate PCR approach to conserved domains from the 12 Drosophila species, we have identified the first reaper and hid orthologs from a tephritid, the Caribfly Anastrepha suspensa. As-hid is the first identified non-drosophilid homolog of hid, and As-rpr is the second non-drosophilid rpr homolog. Both genes share more than 50% amino acid sequence identity with their Drosophila homologs, suggesting that insect pro-apoptotic peptides may be more conserved than previously anticipated. Importantly, both genes encode the conserved IBM and GH3 motifs that are key for IAP-inhibition and mitochondrial localization. Functional verification of both genes as cell death effectors was demonstrated by cell death assays in A. suspensa embryonic cell culture, as well as in heterologous Drosophila melanogaster S2 cells. Notably, heterologous cell death activity was found to be higher for Anastrepha genes than their Drosophila counterparts. In common with the Drosophila cognates, As-hid and As-rpr negatively regulated the Drosophila inhibitor of apoptosis (DIAP1) gene to promote apoptosis, and both genes when used together effected increased cell death activity, indicating a co-operative function for As-hid and As-rpr. We show that these tephritid cell death genes are functional and potent as cell death effectors, and could be used to design improved transgenic lethality systems for insect population control.
机译：由于其对凋亡抑制因子（IAP）的拮抗作用，来自收割者，隐藏，严峻（RHG）家族的促凋亡蛋白是果蝇程序性细胞死亡的主要调节剂，从而释放了影响凋亡的胱天蛋白酶的IAP抑制作用。使用简并PCR方法从12个果蝇物种的保守域，我们已经确定了第一个收割者和隐藏的直系同源物，从一个象甲虫，Caribfly Anastrepha suspensa。 As-hid是hid的第一个鉴定出的非果蝇同源物，As-rpr是第二个非嗜果的rpr同源物。这两个基因与其果蝇同源物共享超过50％的氨基酸序列同一性，表明昆虫促凋亡肽可能比以前预期的更为保守。重要的是，这两个基因均编码保守的IBM和GH3基序，这对IAP抑制和线粒体定位至关重要。这两种基因作为细胞死亡效应子的功能验证已通过在A.suspensa胚胎细胞培养物中以及在异源果蝇S2细胞中进行的细胞死亡试验得以证实。值得注意的是，发现Anastrepha基因的异源细胞死亡活性高于果蝇对应物。与果蝇认识相同，As-hid和As-rpr负调节果蝇凋亡抑制剂（DIAP1）基因以促进细胞凋亡，并且两个基因一起使用时会增加细胞死亡活性，表明As-协同作用隐藏和As-rpr。我们表明，这些死球细胞死亡基因是功能强大的细胞死亡效应子，可用于设计改良的转基因杀虫系统以控制昆虫种群。

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