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The Effect of Wi-Fi Radiation on the Mineralization and Oxidative Stress of Osteoblasts

机译:Wi-Fi辐射对成骨细胞矿化和氧化应激的影响

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Some articles reported that Wi-Fi radiation induced oxidative stress (OS) in cells and vital organs. However, the possible effects of Wi-Fi electromagnetic fields (EMFs) on bone cells have not yet been investigated. MC3T3-E1 cells were cultured in cell incubators during induced differentiation and placed 3 cm from Wi-Fi antenna. A 2.45-GHz Wi-Fi signal, transmitted between a Wi-Fi router and a laptop Wi-Fi antenna, radiated on cells for 30 min/day over a 7-day period. The two modes of the Wi-Fi irradiation were 100 mW and 500 mW. The specific absorption rates (SARs) in the cell layer by 100 mW and 500 mW Wi-Fi were 0.1671 W/kg and 0.8356 W/kg, respectively, represented as SARa and SARb, and the cell layer temperature increased by 0.065 degrees C and 0.32 degrees C, respectively, after 30 min of irradiation by finite difference-time domain (FDTD) simulation. The cell oxidative stress indexes were measured by a microplate reader, and the calcified nodules were examined by alizarin red S staining. At a 3-cm close range, 2.45-GHz Wi-Fi radiation increased Reactive oxygen species (ROS) and glutathione (GSH) levels in osteoblasts with the increase of irradiation time, and the quantity of mineralization slightly depended on the radiation intensity.
机译:有些文章报告了Wi-Fi辐射诱导细胞和重要器官中的氧化应激(OS)。然而,尚未研究Wi-Fi电磁场(EMF)对骨细胞的可能影响。在诱导的分化期间在细胞培养箱中培养MC3T3-E1细胞,并从Wi-Fi天线置于3厘米。 2.45GHz Wi-Fi信号,在Wi-Fi路由器和笔记本电脑Wi-Fi天线之间传输,在7天内辐射在电池30分钟/天。 Wi-Fi辐射的两种模式为100 MW和500 MW。细胞层中的比吸收率(SARS)通过100mW和500mW Wi-Fi分别为0.1671W / kg和0.8356W / kg,表示为Sara和Sarb,电池层温度增加0.065℃通过有限差分时域(FDTD)模拟30分钟照射后,分别为0.32℃。通过微孔板读数器测量细胞氧化应激指数,并通过茜素红S染色检查钙化结节。在3厘米的近距离范围内,2.45GHz Wi-Fi辐射在辐照时间的增加增加,成骨细胞中的反应性氧物质(ROS)和谷胱甘肽(GSH)水平,矿化量略微依赖于辐射强度。

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