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首页> 外文期刊>Applied Mathematical Modelling >Characterisation of stresses on microcarriers in a stirred bioreactor
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Characterisation of stresses on microcarriers in a stirred bioreactor

机译：搅拌生物反应器中微载体上的应力表征

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摘要

A computational fluid dynamics model for the flow of culture in a Corning™ spinner-flask stirred bioreactor has been used to characterise stresses experienced by microcarriers immersed in the fluid. Validation of the turbulent flow using experimental Particle Image Velocimetry (PIV) found advanced Large Eddy Simulation (LES) to be superior to Unsteady Reynolds averaging (URANS) modelling as a computational strategy for accurately capturing instantaneous velocity fluctuations of the types observed in the experiments. The simulations demonstrated that stress exposures experienced by microcarriers were highest during impeller start-up. After start-up, microcarriers experienced elevated levels of fluctuating stress of magnitudes known to cause cell differentiation, potentially compromising expansion of a homogeneous population with multi-lineage potential. Decreasing the impeller speed from 70 RPM to 50 RPM in the Corning™ flask was not found to necessarily reduce microcarrier stress exposure, because such a measure does not control the spatio-temporal coincidence of the microcarrier population with high regions of stress within the bioreactor. Modifications to bioreactor geometries and operational protocols are identified that can be pursued if such issues with dynamic stem cell culture arise.
机译：用于Corning™旋转烧瓶搅拌生物反应器中培养物流动的计算流体动力学模型已用于表征浸没在流体中的微载体所承受的应力。使用实验粒子图像测速（PIV）进行的湍流验证发现，先进的大涡模拟（LES）优于非稳态雷诺平均（URANS）建模，因为该计算策略可准确捕获实验中观察到的瞬时速度波动。仿真表明，在叶轮启动过程中，微载体承受的应力最大。启动后，微载体经历的波动应力水平升高，已知会导致细胞分化，其大小可能会损害具有多谱系潜能的同质种群的扩张。在康宁™烧瓶中，未将叶轮速度从70 RPM降低到50 RPM并不一定会减少微载体应力暴露，因为这样的措施不能控制生物反应器内具有高应力区域的微载体群体的时空一致性。如果对动态干细胞培养产生此类问题，可以对生物反应器的几何形状和操作规程进行修改。

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  • 来源
    《Applied Mathematical Modelling》 |2016年第16期|6787-6804|共18页
  • 作者

    J.D. Berry; P. Liovic; I.D. Sutalo; R.L. Stewart; V. Glattauer; L. Meagher;

  • 作者单位

    Commonwealth Scientific and Industrial Research Organisation (CSIRO) Mineral Resources, Clayton, Victoria 3168, Australia ,University of Melbourne Chemical & Blomolecular Engineering University of Melbourne Parkville, 3010 Australia;

    Commonwealth Scientific and Industrial Research Organisation (CSIRO) Mineral Resources, Clayton, Victoria 3168, Australia;

    CSIRO Manufacturing, Highett, Victoria 3190, Australia ,Centre for Population Health Research, Faculty of Health Sciences, Curtin University, Perth, Western Australia 6845, Australia;

    CSIRO Manufacturing, Highett, Victoria 3190, Australia;

    CSIRO Manufacturing, Clayton, Victoria 3168, Australia;

    CSIRO Manufacturing, Clayton, Victoria 3168, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Computational fluid dynamics; Stem cells; Suspension bioreactor; Microcarriers; Shear stress;

    机译：计算流体动力学;干细胞;悬浮生物反应器;微载体;剪应力;

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