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首页> 外文期刊>Applied Microbiology and Biotechnology >Field application of nitrogen and phenylacetylene to mitigate greenhouse gas emissions from landfill cover soils: effects on microbial community structure
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Field application of nitrogen and phenylacetylene to mitigate greenhouse gas emissions from landfill cover soils: effects on microbial community structure

机译：氮和苯乙炔在野外的应用，以减轻填埋场覆盖土壤的温室气体排放：对微生物群落结构的影响

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摘要

Landfills are large sources of CH4, but a considerable amount of CH4 can be removed in situ by methanotrophs if their activity can be stimulated through the addition of nitrogen. Nitrogen can, however, lead to increased N2O production. To examine the effects of nitrogen and a selective inhibitor on CH4 oxidation and N2O production in situ, 0.5 M of NH4Cl and 0.25 M of KNO3, with and without 0.01% (w/v) phenylacetylene, were applied to test plots at a landfill in Kalamazoo, MI from 2007 November to 2009 July. Nitrogen amendments stimulated N2O production but had no effect on CH4 oxidation. The addition of phenylacetylene stimulated CH4 oxidation while reducing N2O production. Methanotrophs possessing particulate methane monooxygenase and archaeal ammonia-oxidizers (AOAs) were abundant. The addition of nitrogen reduced methanotrophic diversity, particularly for type I methanotrophs. The simultaneous addition of phenylacetylene increased methanotrophic diversity and the presence of type I methanotrophs. Clone libraries of the archaeal amoA gene showed that the addition of nitrogen increased AOAs affiliated with Crenarchaeal group 1.1b, while they decreased with the simultaneous addition of phenylacetylene. These results suggest that the addition of phenylacetylene with nitrogen reduces N2O production by selectively inhibiting AOAs and/or type II methanotrophs.
机译：垃圾填埋场是CH 4 的主要来源，但是如果可以通过添加氮来激发甲烷的活性，则可以通过甲烷营养生物原位去除大量的CH 4 。但是，氮可以导致N 2 O产量增加。研究氮和选择性抑制剂对CH 4 氧化和N 2 O原位生成，0.5 M NH 4 Cl和从2007年11月至2009年7月，在密歇根州卡拉马祖的一个垃圾填埋场中，将0.25 M的KNO 3 （含和不含0.01％（w / v）苯乙炔）用于试验区。氮的添加促进了N 2 O的产生，但对CH 4 的氧化没有影响。苯乙炔的加入促进了CH 4 的氧化，同时减少了N 2 O的产生。具有微粒甲烷单加氧酶的甲烷营养菌和古细菌氨氧化剂（AOA）丰富。氮的添加降低了甲烷营养的多样性，特别是对于I型甲烷营养。苯乙炔的同时加入增加了甲烷营养的多样性和I型甲烷营养的存在。古细菌amoA基因的克隆文库显示，氮的添加增加了与Crnarchaeal组1.1b相关的AOA，而氮的减少则同时添加了苯乙炔。这些结果表明，通过选择性地抑制AOAs和/或II型甲烷营养生物，在氮中加入苯乙炔可降低N 2 O的产生。

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