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首页> 外文期刊>Applied Microbiology and Biotechnology >Phylogenetic and functional diversity of propionate-oxidizing bacteria in an anaerobic digester sludge
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Phylogenetic and functional diversity of propionate-oxidizing bacteria in an anaerobic digester sludge

机译：厌氧消化池污泥中丙酸氧化细菌的系统发育和功能多样性

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摘要

The phylogenetic and functional diversity of syntrophic propionate-oxidizing bacteria (POB) present in an anaerobic digester was investigated by microautoradiography combined with fluorescent in situ hybridization (MAR–FISH) that can directly link 16S rRNA phylogeny with in situ metabolic function. The syntrophic POB community in the anaerobic digester sludge consisted of at least four phylogenetic groups: Syntrophobacter, uncultured short rod Smithella (Smithella sp. SR), uncultured long rod Smithella (Smithella sp. LR), and an unidentified group. The activities of these POB groups were dependent on the propionate concentrations. The uncultured Smithella sp. SR accounted for 52–62% of the total active POB under all the propionate concentrations tested (0.5–15 mM). In contrast, uncultured Smithella sp. LR was active only at lower propionate concentrations and became a dominant active POB at 0.5 mM of propionate. Syntrophobacter accounted for 16–31% of the total active POB above 2.5 mM propionate, whereas the active Syntrophobacter population became low (ca. 6%) at 0.5 mM of propionate. The anaerobic digester was operated in a fill and draw mode, resulting in periodical changes in propionate concentration ranging from 0 to 10 mM. These phylogenetically and functionally diverse, to some extent functionally redundant, active POB communities were dynamically responding to the periodical changes in propionate concentration.
机译：通过微放射自显影结合荧光原位杂交（MAR-FISH）研究了厌氧消化池中的营养性丙酸氧化细菌（POB）的系统发育和功能多样性，该技术可以直接将16S rRNA系统发育与原位代谢功能联系起来。厌氧消化池污泥中的同养型POB群落至少由四个系统发育组组成：滋养细菌，未培养的短杆史密斯菌（Smithella sp。SR），未培养的长杆史密斯菌（Smithella sp。LR）和一个身份不明的群体。这些POB组的活性取决于丙酸酯浓度。未经养殖的史密斯菌。在所有测试的丙酸酯浓度（0.5-15 mM）下，SR占总活性POB的52-62％。相反，未培养的Smithella sp。 LR仅在较低的丙酸酯浓度下才有活性，并在0.5 mM的丙酸酯中成为主要的活性POB。高于2.5 mM的丙酸时，滑膜细菌占总活性POB的16-31％，而当丙酸为0.5 mM时，活性滑膜细菌数量变少（约6％）。厌氧消化池以填充和抽取模式运行，导致丙酸盐浓度在0到10 mM之间周期性变化。这些系统发育和功能上的多样性，某种程度上在功能上是冗余的，活跃的POB群落动态地响应于丙酸浓度的周期性变化。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2007年第3期|673-683|共11页
  • 作者

    Herto Dwi Ariesyady; Tsukasa Ito; Kazumi Yoshiguchi; Satoshi Okabe;

  • 作者单位

    Department of Urban and Environmental Engineering Graduate School of Engineering Hokkaido University North-13 West-8 Kita-ku Sapporo 060-8628 Japan;

    Department of Civil Engineering Faculty of Engineering Gunma University 1-5-1 Tenjin-cho Kiryu 376-8515 Japan;

    Department of Urban and Environmental Engineering Graduate School of Engineering Hokkaido University North-13 West-8 Kita-ku Sapporo 060-8628 Japan;

    Department of Urban and Environmental Engineering Graduate School of Engineering Hokkaido University North-13 West-8 Kita-ku Sapporo 060-8628 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Anaerobic digestion; Propionate-oxidizing bacteria; MAR–FISH; Functional diversity;

    机译：厌氧消化;丙酸氧化细菌;MAR–FISH;功能多样性;

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