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首页> 外文期刊>Applied Microbiology and Biotechnology >Sequencing of the intergenic 16S-23S rRNA spacer (ITS) region of Mollicutes species and their identification using microarray-based assay and DNA sequencing
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Sequencing of the intergenic 16S-23S rRNA spacer (ITS) region of Mollicutes species and their identification using microarray-based assay and DNA sequencing

机译：Mollicutes物种的基因间16S-23S rRNA间隔区（ITS）区域的测序及其使用基于微阵列的分析和DNA测序的鉴定

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摘要

We have completed sequencing the 16S-23S rRNA intergenic transcribed spacer (ITS) region of most known Mycoplasma , Acholeplasma , Ureaplasma , Mesoplasma , and Spiroplasma species. Analysis of the sequence data revealed a significant interspecies variability and low intraspecies polymorphism of the ITS region among Mollicutes . This finding enabled the application of a combined polymerase chain reaction–microarray technology for identifying Mollicutes species. The microarray included individual species-specific oligonucleotide probes for characterizing human Mollicutes species and other species known to be common cell line contaminants. Evaluation of the microarray was conducted using multiple, previously characterized, Mollicutes species. The microarray analysis of the samples used demonstrated a highly specific assay, which is capable of rapid and accurate discrimination among Mollicutes species.
机译：我们已经完成了对最广为人知的支原体，无形体，尿素体，中性体和螺旋体物种的16S-23S rRNA基因转录间隔区（ITS）区域的测序。对序列数据的分析显示，Mollicutes的ITS区存在明显的种间变异性和低种间多态性。这一发现使得能够应用聚合酶链反应-微阵列组合技术鉴定Mollicutes种类。该微阵列包括用于表征人类Mollicutes物种和已知为常见细胞系污染物的其他物种的单个物种特异性寡核苷酸探针。使用多个先前表征的Mollicutes物种对微阵列进行评估。对所用样品的微阵列分析显示出高度特异性的测定方法，该方法能够快速准确地区分Mollicutes菌种。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2006年第5期|680-698|共19页
  • 作者

    Dmitriy V. Volokhov; Joseph George; Sue X. Liu; Pranvera Ikonomi; Christine Anderson; Vladimir Chizhikov;

  • 作者单位

    Center of Biologics Evaluation and Research Office of Vaccines Research and Review Division of Viral Products Laboratory of Methods Development US Food and Drug Administration;

    Center of Biologics Evaluation and Research Office of Vaccines Research and Review Division of Viral Products Laboratory of Methods Development US Food and Drug Administration;

    Center of Biologics Evaluation and Research Office of Vaccines Research and Review Division of Viral Products Laboratory of Methods Development US Food and Drug Administration;

    American Type Culture Collection (ATCC);

    Center of Biologics Evaluation and Research Office of Vaccines Research and Review Division of Viral Products Laboratory of Methods Development US Food and Drug Administration;

    Center of Biologics Evaluation and Research Office of Vaccines Research and Review Division of Viral Products Laboratory of Methods Development US Food and Drug Administration;

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