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首页> 外文期刊>Applied Microbiology and Biotechnology >Regioselective hydroxylation of quinolinic acid, lutidinic acid and isocinchomeronic acid by resting cells of pyridine dicarboxylic acid-degrading microorganisms
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Regioselective hydroxylation of quinolinic acid, lutidinic acid and isocinchomeronic acid by resting cells of pyridine dicarboxylic acid-degrading microorganisms

机译：吡啶二羧酸降解微生物的静息细胞对喹啉酸，戊二酸和异辛烯酸的区域选择性羟基化

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摘要

Microorganisms aerobically degrading quinolinic acid, lutidinic acid or isocinchomeronic acid were isolated and the microbial regioselective hydroxylation of these pyridine dicarboxylic acids was studied. Alcaligenes sp. UK21 cells converted quinolinic acid into 6-hydroxypicolinic acid, suggesting the involvement of two enzyme reactions catalyzing hydroxylation at position C6 and decarboxylation at position C3 of quinolinic acid. Resting cells of Alcaligenes sp. UK21 accumulated 94.9 mM 6-hydroxypicolinic acid (13.2 g l−1), with a 96% molar conversion yield by 48 h incubation. Rhizobium sp. LA17 and Hydrogenophaga sp. IMA01 catalyzed the regioselective hydroxylation of lutidinic acid and isocinchomeronic acid into 6-hydroxylutidinic acid and 6-hydroxyisocinchomeronic acid, respectively. 6-Hydroxylutidinic acid accumulated up to 95.4 mM (17.5 g l−1) by 24 h incubation in the resting cells reaction, using Rhizobium sp. LA17, with a 99% molar conversion yield. Resting cells of Hydrogenophaga sp. IMA01 produced 88.7 mM 6-hydroxyisocinchomeronic acid (16.2 g l−1) by 24 h incubation, with a 81% molar conversion yield.
机译：分离出需氧降解喹啉酸，二氢吡啶二酸或异辛烯酸的微生物，并研究了这些吡啶二羧酸的微生物区域选择性羟基化。产碱菌UK21细胞将喹啉酸转化为6-羟基吡啶甲酸，表明参与了两个催化喹啉酸C6羟基化和C3羧基脱羧的酶反应。产碱杆菌的静止细胞。 UK21在48 h孵育过程中积累了94.9 mM的6-羟基吡啶甲酸（13.2 g l-1 ），摩尔转化率为96％。根瘤菌LA17和Hydrogenophaga sp。 IMA01分别将戊二酸和异辛香烯酸的区域选择性羟基化反应生成6-羟基二丁烯二酸和6-羟基异辛香烯酸。使用根瘤菌（Rhizobium sp。）在静息细胞反应中温育24 h，6-羟基二氢吡啶二酸累积达95.4 mM（17.5 g l-1 ）。 LA17，具有99％的摩尔转化率。 Hydrogenophaga sp。的静息细胞。通过孵育24小时，IMA01产生了88.7 mM的6-羟基异辛烯酸（16.2 g l-1 ），摩尔转化率为81％。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2003年第4期|337-341|共5页
  • 作者

    A. Uchida; T. Yoshida; M. Ogawa; T. Nagasawa;

  • 作者单位

    Department of Biomolecular Science Gifu University;

    Department of Biomolecular Science Gifu University;

    Department of Biomolecular Science Gifu University;

    Department of Biomolecular Science Gifu University;

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