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Microbial diversity of a mesophilic hydrogen-producing sludge

机译：中温产氢污泥的微生物多样性

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摘要

A hydrogen-producing sludge degraded 99% of glucose at 36 °C and pH 5.5, producing a methane-free biogas (comprising 64% hydrogen) and an effluent comprising mostly butyrate, acetate, and ethanol. The yield was 0.26 l H2 g–1 glucose and the production rate per gram of volatile suspended solids was 4.6 l H2 day–1. A 16S rDNA library was constructed from the sludge for microbial species determination. A total of 96 clones were selected for plasmids recovery, screened by denaturing gradient gel electrophoresis, and sequenced for rDNA. Based on the phylogenetic analysis of the rDNA sequences, 64.6% of all the clones were affiliated with three Clostridium species (Clostridiaceae), 18.8% with Enterobacteriaceae, and 3.1% with Streptococcus bovis (Streptococcaceae). The remaining 13.5% belonged to eight operational taxonomic units, the affiliations of which were not identified.
机译：产生氢气的污泥在36°C和pH 5.5时降解了99％的葡萄糖，产生了无甲烷的沼气（包含64％的氢气）和主要包含丁酸酯，乙酸盐和乙醇的废水。产量为0.26 l H2 g-1 葡萄糖，每克挥发性悬浮固体的生产率为4.6 l H2 day-1 。从污泥中构建了一个16S rDNA文库，用于微生物种类的测定。总共选择了96个克隆用于质粒回收，通过变性梯度凝胶电泳进行筛选，并对rDNA进行测序。根据rDNA序列的系统发育分析，所有克隆中有64.6％与3个梭菌属（Clostridiaceae），肠杆菌科（Enterobacteriaceae）和18.8％与牛链球菌（Streptococcus bovis）（链球菌）相关。其余的13.5％属于八个运营生物分类单位，但未确定其隶属关系。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2002年第1期|112-118|共7页
  • 作者

    H. Fang; T. Zhang; H. Liu;

  • 作者单位

    Centre for Environmental Engineering Research Department of Civil Engineering The University of Hong Kong Pokfulam Road Hong Kong SAR China;

    Centre for Environmental Engineering Research Department of Civil Engineering The University of Hong Kong Pokfulam Road Hong Kong SAR China;

    Centre for Environmental Engineering Research Department of Civil Engineering The University of Hong Kong Pokfulam Road Hong Kong SAR China;

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