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首页> 外文期刊>Applied Microbiology and Biotechnology >Molecular detection and diversity of polycyclic aromatic hydrocarbon-degrading bacteria isolated from geographically diverse sites
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Molecular detection and diversity of polycyclic aromatic hydrocarbon-degrading bacteria isolated from geographically diverse sites

机译：从不同地理位置分离的多环芳烃降解细菌的分子检测和多样性

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Nineteen polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were isolated from environmental samples in Kuwait, Indonesia, Thailand, and Japan by enrichment with either naphthalene or phenanthrene as a sole carbon source. Sequence analyses of the 16-S rRNA gene indicated that at least seven genera (Ralstonia, Sphingomonas, Burkholderia, Pseudomonas, Comamonas, Flavobacterium, and Bacillus) were present in this collection. Determination of the ability of the isolates to use PAH and its presumed catabolic intermediates suggests that the isolates showed multiple phenotypes in terms of utilization and degradation pathways. The large subunit of the terminal oxygenase gene (phnAc) from Burkholderia sp. strain RP007 hybridized to 32% (6/19) of the isolates, whilst gene probing using the large subunit of terminal oxygenase gene (pahAc) from Pseudomonas putida strain OUS82 revealed no pahAc-like genes amongst the isolates. Using three degenerated primer sets (pPAH-F/NR700, AJ025/26, and RieskeF/R), targeting a conserved region with the genes encoding the large subunit of terminal oxygenase successfully amplified material from 6 additional PAH-degrading isolates. Sequence analyses showed that the large subunit of terminal oxygenase in 4 isolates was highly homologous to the large subunit of naphthalene dioxygenase gene from Ralstonia sp. strain U2. However, we could not obtain any information on the oxygenase system involved in the naphthalene and/or phenathrene degradation by 7 other strains. These results suggest that PAH-degrading bacteria are diverse, and that there are still many unidentified PAH-degrading bacteria.
机译：通过富集萘或菲作为唯一碳源，从科威特，印度尼西亚，泰国和日本的环境样品中分离出19种降解多环芳烃（PAH）的细菌。 16-S rRNA基因的序列分析表明，在该集合中至少存在七个属（罗氏菌，鞘氨醇单胞菌，伯克霍尔德氏菌，假单胞菌，Comamonas，黄杆菌和芽孢杆菌）。确定分离物使用PAH及其推测的分解代谢中间体的能力表明，分离物在利用和降解途径方面表现出多种表型。伯克霍尔德氏菌（Burkholderia sp。）的末端加氧酶基因（phnAc）的大亚基。 RP007菌株与32％（6/19）的菌株杂交，同时使用恶臭假单胞菌OUS82菌株末端加氧酶基因（pahAc）大亚基进行基因探测时，菌株中未发现pahAc样基因。使用三个简并引物组（pPAH-F / NR700，AJ025 / 26和RieskeF / R），用编码末端加氧酶大亚基的基因靶向保守区域，成功地从6个另外的降解PAH的分离物中扩增了材料。序列分析表明，在4个分离物中，末端加氧酶的大亚基与Ralstonia sp。的萘双加氧酶基因的大亚基高度同源。菌株U2。但是，我们无法获得有关其他7种菌株降解萘和/或菲萘斯的氧化酶系统的任何信息。这些结果表明，降解PAH的细菌是多种多样的，并且仍然存在许多未鉴定的降解PAH的细菌。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2002年第2期|202-209|共8页
  • 作者

    J. Widada; H. Nojiri; K. Kasuga; T. Yoshida; H. Habe; T. Omori;

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    Biotechnology Research Center The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113-8657 Japan;

    Biotechnology Research Center The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113-8657 Japan;

    Department of Biotechnology Faculty of Bioresource Sciences Akita Prefectural University 241-7 Kaidobata-nishi Shimoshinjo-nakano Akita-shi Akita 010-0195 Japan;

    Biotechnology Research Center The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113-8657 Japan;

    Biotechnology Research Center The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113-8657 Japan;

    Biotechnology Research Center The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113-8657 Japan;

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