...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology and Biotechnology >Inhibition of bacterial α-glucosidases by castanospermine in pure cultures and activated sludge
【24h】

Inhibition of bacterial α-glucosidases by castanospermine in pure cultures and activated sludge

机译：粟精胺在纯培养物和活性污泥中对细菌α-葡萄糖苷酶的抑制作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Castanospermine (CAST) is a known and potent inhibitor of various α-glucosidases in eukaryotes. In this work, we elucidated whether CAST could also be used for determining bacterial α-glucosidase activity, when measured with 4-methylumbelliferyl-α-D-glucoside as a substrate, both in a complex bacterial community, in activated sludge and in pure cultures of bacterial isolates. We found that 140 µM CAST inhibited α-glucosidase activity by 30% in a pure culture of Pseudomonas stutzeri. The α-glucosidase activity in Chryseobacterium gleum was inhibited by 90% at a concentration of 150 µM CAST, whereas the α-glucosidase in Paracoccus denitrificans was resistant to the inhibitor. CAST (140 µM) reduced α-glucosidase activity in activated sludge by 40%, the respiration rate being reduced by only 12%. No significant inhibition of the respiration rate was observed in Ps. stutzeri or Pa. denitrificans, whereas the respiration rate in C. gleum grown in a medium containing starch was inhibited by 50% with 140 µM CAST. No effect of CAST was observed in C. gleum grown in a complex medium. This indicated that CAST, at the concentration used, did not cause a general negative effect on bacterial activity. The results suggest that the CAST assay may potentially be useful in determining whether α-glucosidase activity, starch, poly- and disaccharides contribute appreciably to the overall activity of a bacterial community. However, the assay should not be used for quantitative measurements of such activity.
机译：粟精胺（CAST）是真核生物中各种α-葡萄糖苷酶的已知有效抑制剂。在这项工作中，我们阐明了在复杂的细菌群落，活性污泥和纯培养物中，以4-甲基伞形酮-α-D-葡萄糖苷为底物进行测量时，CAST是否也可用于确定细菌α-葡萄糖苷酶活性。细菌分离株。我们发现140 µM CAST在纯净的斯氏假单胞菌培养物中可将α-葡萄糖苷酶活性抑制30％。在浓度为150 µM CAST的情况下，胶质芽孢杆菌中的α-葡萄糖苷酶活性受到90％的抑制，而反硝化副球菌中的α-葡萄糖苷酶则对该抑制剂具有抗性。 CAST（140 µM）使活性污泥中的α-葡萄糖苷酶活性降低40％，呼吸速率仅降低12％。在Ps中未观察到对呼吸速率的显着抑制。 Stutzeri或Denitrificans，而在含淀粉的培养基中生长的C. gleum的呼吸速率被140 µM CAST抑制了50％。在复杂培养基中生长的球状梭菌中未观察到CAST的作用。这表明，在所使用的浓度下，CAST不会对细菌活性产生普遍的负面影响。结果表明，CAST分析可能可用于确定α-葡萄糖苷酶活性，淀粉，多糖和二糖是否对细菌群落的整体活性有明显贡献。但是，该测定法不应用于此类活性的定量测量。

著录项

  • 来源
    《Applied Microbiology and Biotechnology》 |2002年第1期|68-71|共4页
  • 作者

    N. Mustafa; M. Thörn; F. Sörensson;

  • 作者单位

    Department of Cell and Molecular biology Microbiology Göteborg University P.O. Box 462 40530 Göteborg Sweden;

    Department of Cell and Molecular biology Microbiology Göteborg University P.O. Box 462 40530 Göteborg Sweden;

    Department of Cell and Molecular biology Microbiology Göteborg University P.O. Box 462 40530 Göteborg Sweden;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号