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首页> 外文期刊>Applied Microbiology and Biotechnology >Study of two-stage processes for the microbial production of 1,3-propanediol from glucose
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Study of two-stage processes for the microbial production of 1,3-propanediol from glucose

机译：从葡萄糖微生物生产1,3-丙二醇的两步法研究

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摘要

The microbial production of 1,3-propanediol (1,3-PD) from glucose was studied in a two-stage fermentation process on a laboratory scale. In the first stage, glucose was converted to glycerol either by the osmotolerant yeast Pichia farinosa or by a recombinant Escherichia coli strain. In the second stage, glycerol in the broth from the first stage was converted to 1,3-PD by Klebsiella pneumoniae. The culture broth from P. farinosa was shown to contain toxic metabolites that strongly impair the growth of K. pneumoniae and the formation of 1,3-PD. Recombinant E. coli is more suitable than P. farinosa for producing glycerol in the first stage. The fermentation pattern from glycerol can be significantly altered by the presence of acetate, leading to a significant reduction of PD yield in the second stage. However, in the recombinant E. coli culture acetate formation can be prevented by fed-batch cultivation under limiting glucose supply, resulting in an effective production of 1,3-PD in the second stage with a productivity of 2.0 g l–1 h–1 and a high yield (0.53 g/g) close to that of glycerol fermentation in a synthetic medium. The overall 1,3-PD yield from glucose in the two stage-process with E. coli and K. pneumoniae reached 0.17 g/g.
机译：在实验室规模的两阶段发酵过程中研究了葡萄糖从微生物产生的1,3-丙二醇（1,3-PD）。在第一阶段，通过耐渗透酵母曲霉毕赤酵母或重组大肠杆菌菌株将葡萄糖转化为甘油。在第二阶段，来自第一阶段的肉汤中的甘油被肺炎克雷伯菌转化为1，3-PD。研究表明，来自粉状疟原虫的培养液含有有毒代谢产物，这些代谢产物强烈损害肺炎克雷伯菌的生长和1,3-PD的形成。重组大肠杆菌比P. farinosa更适合在第一阶段生产甘油。乙酸盐的存在可以显着改变甘油的发酵模式，从而导致第二阶段的PD产量显着降低。但是，在重组大肠杆菌培养物中，通过限制葡萄糖供应的补料分批培养可以防止乙酸盐的形成，从而在第二阶段有效生产1,3-PD，生产力为2.0 gl-1 h–1 ，在合成培养基中的收率接近甘油发酵的高收率（0.53 g / g）。在大肠杆菌和肺炎克雷伯菌的两个阶段中，葡萄糖的总1,3-PD产量达到0.17 g / g。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2002年第2期|60-66|共7页
  • 作者

    M. Hartlep; W. Hussmann; N. Prayitno; I. Meynial-Salles; A.-P. Zeng;

  • 作者单位

    Gesellschaft für Biotechnologische Forschung mbH Biochemical Engineering Division Mascheroder Weg 1 38124 Braunschweig Germany;

    Gesellschaft für Biotechnologische Forschung mbH Biochemical Engineering Division Mascheroder Weg 1 38124 Braunschweig Germany;

    Gesellschaft für Biotechnologische Forschung mbH Biochemical Engineering Division Mascheroder Weg 1 38124 Braunschweig Germany;

    CRITT-Bioindustries CRT INSA DGBA 135 Avenue de Rangueil 31077 Toulouse cedex4 France;

    Gesellschaft für Biotechnologische Forschung mbH Biochemical Engineering Division Mascheroder Weg 1 38124 Braunschweig Germany;

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