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首页> 外文期刊>Applied Microbiology and Biotechnology >Detection of two distinct substrate-dependent catabolic responses in yeast cells using a mediated electrochemical method
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Detection of two distinct substrate-dependent catabolic responses in yeast cells using a mediated electrochemical method

机译：使用介导的电化学方法检测酵母细胞中两种不同的底物依赖性分解代谢反应

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摘要

Mediated electrochemical detection of catabolism in prokaryotic cells is well documented; however, the application of this technique to eukaryotic cells has received less attention. Two catabolic substrate-dependent mediated electrochemical signals were detected in the yeast Saccharomyces cerevisiae. The signal using a single hydrophilic mediator (ferricyanide) is small whereas the response using a double mediator system comprising a hydrophilic and a lipophilic mediator (ferricyanide and menadione) is up to three orders of magnitude larger. The behaviour of each response during cell ageing is different: the single mediator response increases whereas the double mediator response decreases. This difference indicates that the two signals originate at different points in the catabolic pathways. In S. cerevisiae the double mediator response is proposed to originate from the reduction of the lipophilic mediator by NADPH produced in the pentose phosphate pathway. The single mediator signal arises from reduction of the hydrophilic mediator by an extracellular redox species produced in response to the presence of glucose.
机译：介导的电化学检测原核细胞中分解代谢的方法已有大量文献报道；然而，该技术在真核细胞中的应用受到的关注较少。在酵母酿酒酵母中检测到两个分解代谢物依赖的介导的电化学信号。使用单个亲水性介体（铁氰化物）的信号很小，而使用包含亲水性和亲脂性介体（铁氰化物和甲萘醌）的双重介体系统的响应则大了三个数量级。在细胞衰老过程中，每种反应的行为都不同：单一介体反应增加，而双重介体反应减少。这种差异表明这两个信号起源于分解代谢途径中的不同点。在酿酒酵母中，双重介体反应被提出是由戊糖磷酸途径中产生的NADPH对亲脂性介体的还原引起的。单个介体信号来自响应于葡萄糖的存在而产生的细胞外氧化还原物质对亲水性介体的还原。

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    《Applied Microbiology and Biotechnology》 |2002年第2期|108-113|共6页
  • 作者

    K. Baronian; A. Downard; R. Lowen; N. Pasco;

  • 作者单位

    School of Applied Science Christchurch Polytechnic Institute of Technology P.O. Box 540 Christchurch New Zealand;

    Department of Chemistry University of Canterbury Private Bag 4800 Christchurch New Zealand;

    School of Applied Science Christchurch Polytechnic Institute of Technology P.O. Box 540 Christchurch New Zealand;

    Lincoln Ventures Ltd Lincoln University PO Box 84 Canterbury New Zealand;

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