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首页> 外文期刊>Applied Microbiology and Biotechnology >Application of high performance anion exchange chromatography to study invertase-catalysed hydrolysis of sucrose and formation of intermediate fructan products
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Application of high performance anion exchange chromatography to study invertase-catalysed hydrolysis of sucrose and formation of intermediate fructan products

机译：高效阴离子交换色谱在蔗糖转化酶催化下水解及中间果聚糖产物形成中的应用

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摘要

Baker's yeast invertase was found to catalyse transfructosylation reactions in aqueous and anhydrous organic media with sucrose as a substrate, leading to the formation of five intermediate fructans in addition to the release of D-glucose (D-Glc)and D-fructose (D-Fru). All the reaction products were separated and quantitatively estimated using high performance anion exchange-pulsed amperometric detection equipment. The unknown products were subsequently identified by linkage analysis as β-D-Fru-(2→1)-β-D-Fru-(2→1)-α-D-glucopyranoside (1-kestose), β-D-Fru-(2→6)-α-D-glucopyranoside (6-β-fructofuranosylglucose), β-D-Fru-(2→1)-β-D-fructofuranoside (inulobiose), β-D-Fru-(2→6)-β-D-Fru-(2→1)-α-D-glucopyranoside (6-kestose) and β-D-Fru-(2→6)-α-D-Glc-(1→2)-β-D-fructofuranoside (neokestose); and this last was eluted together with a disaccharide. The time-course of sucrose hydrolysis via fructan production in 2 ml of a 50 mM sodium acetate buffer (pH 4.5) containing 0.2 M sucrose and 25 U of invertase was different from that in 2 ml of anhydrous toluene with 1.46 M sucrose and 1,000 U of invertase as a suspended powder. Under the latter experimental conditions, invertase was found to exhibit cyclic behaviour, where sucrose was degraded and subsequently synthesised. This observation has not yet been reported, as far as we know.
机译：发现贝克酵母转化酶可以在水和无水有机介质中以蔗糖为底物催化反果糖基化反应，除释放D-葡萄糖（D-Glc）和D-果糖（D- Fru）。使用高性能阴离子交换脉冲安培检测设备分离并定量估计所有反应产物。随后通过连锁分析将未知产物鉴定为β-D-Fru-（2→1）-β-D-Fru-（2→1）-α-D-吡喃葡萄糖苷（1-koseose），β-D-Fru -（2→6）-α-D-吡喃葡糖苷（6-β-果糖呋喃糖基葡萄糖），β-D-Fru-（2→1）-β-D-果糖呋喃糖苷（吲哚二糖），β-D-Fru-（2→ 6）-β-D-Fru-（2→1）-α-D-吡喃吡喃糖苷（6-koseose）和β-D-Fru-（2→6）-α-D-Glc-（1→2）- β-D-果糖呋喃糖苷（神经糖）；然后将其与二糖一起洗脱。在2 ml的含0.2 M蔗糖和25 U转化酶的50 mM乙酸钠缓冲液（pH 4.5）中通过果糖生产产生的蔗糖水解的时间过程不同于2 ml含1.46 M蔗糖和1,000 U的无水甲苯悬浮粉末形式的转化酶在后一实验条件下，发现转化酶表现出循环行为，其中蔗糖被降解并随后合成。据我们所知，尚未观察到该观察结果。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2001年第1期|55-60|共6页
  • 作者

    S. Farine; C. Versluis; P. Bonnici; A. Heck; C. Lhomme; A. Puigserver; A. Biagini;

  • 作者单位

    Institut Méditerranéen de Recherche en Nutrition UMR Université-INRA Faculté des Sciences et Techniques de Saint-Jérôme service 342 13397 Marseille Cedex 20 France;

    Department of Biomolecular Mass Spectrometry Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences Utrecht University Sorbonnelaan 16 3584 CA Utrecht The Netherlands;

    Department of Biomolecular Mass Spectrometry Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences Utrecht University Sorbonnelaan 16 3584 CA Utrecht The Netherlands;

    Department of Biomolecular Mass Spectrometry Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences Utrecht University Sorbonnelaan 16 3584 CA Utrecht The Netherlands;

    Institut Méditerranéen de Recherche en Nutrition UMR Université-INRA Faculté des Sciences et Techniques de Saint-Jérôme service 342 13397 Marseille Cedex 20 France;

    Institut Méditerranéen de Recherche en Nutrition UMR Université-INRA Faculté des Sciences et Techniques de Saint-Jérôme service 342 13397 Marseille Cedex 20 France;

    Institut Méditerranéen de Recherche en Nutrition UMR Université-INRA Faculté des Sciences et Techniques de Saint-Jérôme service 342 13397 Marseille Cedex 20 France;

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