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首页> 外文期刊>Applied Microbiology and Biotechnology >Characterisation of the microbial 16S rDNA diversity of an aerobic phosphorus-removal ecosystem and monitoring of its transition to nitrate respiration
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Characterisation of the microbial 16S rDNA diversity of an aerobic phosphorus-removal ecosystem and monitoring of its transition to nitrate respiration

机译：需氧除磷生态系统中微生物16S rDNA多样性的表征及其向硝酸盐呼吸的转变的监测

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摘要

The microbial community of a conventional anaerobic–aerobic sequencing batch reactor was investigated by cloning and sequencing bacterial 16S rDNA. The 92 16S rDNA sequences analysed ranged across 50 different operational taxonomic units (OTU). The majority of these sequences were not closely related to known species. They belonged to 12 different groups, but essentially to the Cytophagales and the Proteobacteria beta, which represented 38% and 17% of the retrieved sequences respectively. No OTU numerically outnumbered the others. However, similarities were observed with previous reports on molecular characterisation of phosphorus-accumulating ecosystems, suggesting an enrichment in microorganisms belonging to the Rhodocyclus group. Thereafter, the ability of this anaerobic–aerobic microbial community to accumulate phosphorus with nitrate as its energy source was investigated. The reactor was shifted from anaerobic–aerobic running conditions to anaerobic–anoxic conditions by injection of nitrate; and its microbial community was monitored by PCR-single strand conformation polymorphism (SSCP). The reactor maintained a good phosphorus accumulation and similar SSCP microbial community patterns for a period of 17 days, suggesting that the same microbial community was able to respire both oxygen and nitrate. However, this situation was unstable, since a breakdown in phosphorus accumulation occurred thereafter.
机译：通过对细菌16S rDNA进行克隆和测序，研究了常规厌氧-好氧测序批处理反应器的微生物群落。分析的92个16S rDNA序列跨50个不同的操作分类单位（OTU）。这些序列中的大多数与已知物种没有密切关系。它们属于12个不同的组，但基本上属于细胞噬菌体和变形杆菌β，它们分别代表所检索序列的38％和17％。没有任何OTU在数量上超过其他OTU。但是，与先前有关富磷生态系统分子特征的报道相似，这表明属于红景天类的微生物富集。此后，研究了该厌氧-好氧微生物群落以硝酸盐为能源积累磷的能力。通过注入硝酸盐，反应器从厌氧-好氧运行条件转变为厌氧-缺氧条件。 PCR单链构象多态性（SSCP）监测其微生物群落。该反应器在17天的时间内保持了良好的磷积累和类似的SSCP微生物群落模式，这表明相同的微生物群落能够呼吸氧气和硝酸盐。然而，这种情况不稳定，因为此后发生了磷积累的破坏。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2001年第4期|500-509|共10页
  • 作者

    Patrick Dabert; Bruno Sialve; Jean-Philippe Delgenès; René Moletta; Jean-Jacques Godon;

  • 作者单位

    Laboratoire de Biotechnologie de l'Environnement Institut National de la Recherche Agronomique Avenue des Etangs 11100 Narbonne France;

    Laboratoire de Biotechnologie de l'Environnement Institut National de la Recherche Agronomique Avenue des Etangs 11100 Narbonne France;

    Laboratoire de Biotechnologie de l'Environnement Institut National de la Recherche Agronomique Avenue des Etangs 11100 Narbonne France;

    Laboratoire de Biotechnologie de l'Environnement Institut National de la Recherche Agronomique Avenue des Etangs 11100 Narbonne France;

    Laboratoire de Biotechnologie de l'Environnement Institut National de la Recherche Agronomique Avenue des Etangs 11100 Narbonne France;

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