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首页> 外文期刊>Applied Microbiology and Biotechnology >Isolation of ftsI and murE genes involved in peptidoglycan synthesis from Corynebacterium glutamicum
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Isolation of ftsI and murE genes involved in peptidoglycan synthesis from Corynebacterium glutamicum

机译：谷氨酸棒状杆菌合成肽聚糖的ftsI和murE基因的分离

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摘要

Corynebacterium glutamicum is known to excrete large amounts of L-glutamic acid upon treatment by penicillin. However, the mechanism of L-glutamate overproduction by penicillin treatment is still unknown. A 5.3-kb HindIII fragment was isolated by directly introducing the C. glutamicum (Brevibacterium lactofermentum) ATCC 13869 gene library into the temperature-sensitive Escherichia coli murE mutant and selecting temperature resistant clones. Two open reading frames (ORFs) were found in this fragment: (1) murE, encoding UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-diaminopimelate ligase, and (2) ftsI, encoding septum-peptidoglycan synthetase, one of the targets of penicillin (penicillin-binding protein 3). Both ORFs were involved in peptidoglycan synthesis. Proteins were synthesized from the C. glutamicum murE and ftsI genes, 55 kDa and 73 kDa respectively, in an in vitro protein synthesis system, using E. coli S30 extracts.
机译：已知谷氨酸棒杆菌经青霉素处理后会排泄大量L-谷氨酸。然而，通过青霉素处理的L-谷氨酸过量生产的机制仍是未知的。通过将谷氨酸棒杆菌（乳酸短杆菌）ATCC 13869基因库直接导入温度敏感型大肠杆菌murE突变体并选择耐温克隆，可分离出5.3kb的HindIII片段。在此片段中发现了两个开放阅读框（ORF）：( 1）murE，编码UDP-N-乙酰基muramoyl-L-丙氨酰基-D-谷氨酸：中-二氨基庚二酸酯连接酶，以及（2）ftsI，编码中隔-肽聚糖聚糖合成酶，青霉素的靶标之一（青霉素结合蛋白3）。两种ORF都参与肽聚糖的合成。在体外蛋白质合成系统中，使用大肠杆菌S30提取物从谷氨酸棒杆菌murE和ftsI基因分别合成55 kDa和73 kDa的蛋白质。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2001年第4期|466-470|共5页
  • 作者

    Champika D. Wijayarathna; Masaaki Wachi; Kazuo Nagai;

  • 作者单位

    Department of Bioengineering Tokyo Institute of Technology 4259 Nagatsuta Midori-ku Yokohama 226–8501 Japan;

    Department of Bioengineering Tokyo Institute of Technology 4259 Nagatsuta Midori-ku Yokohama 226–8501 Japan;

    Department of Bioengineering Tokyo Institute of Technology 4259 Nagatsuta Midori-ku Yokohama 226–8501 Japan;

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