Transformation of 2,2′-dichlorodiisopropyl ether in mixed and pure culture

R. Hauck; L. Adrian; P. Wendler; M. Amidjojo; W. Hegemann; H. Görisch

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Transformation of 2,2′-dichlorodiisopropyl ether in mixed and pure culture

机译：混合和纯培养中2,2'-二氯二异丙醚的转化

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An aerobic enrichment culture derived from a groundwater contaminated with organic and chloroorganic compounds was adapted to the transformation of 2,2′-dichlorodiisopropyl ether (DDE) in a continuous fixed-bed bioreactor. Continuous DDE removal efficiencies over 90% were achieved with a model water containing 3.3 mM methanol as co-substrate at DDE loading rates of up to 150 µmol l–1 day–1 with a hydraulic retention time of 24 h. In batch cultures, a stoichiometric release of 2 µmol chloride per µmol DDE transformed was observed. From the mixed culture, a strain was isolated that is able to grow on DDE as the sole energy and carbon source, tolerating DDE concentrations of up to 1 mM. Based on 16S rRNA sequencing, the strain is affiliated with the genus Rhodococcus.

机译：一种源于有机和氯有机化合物污染的地下水的需氧富集培养物适合在连续固定床生物反应器中转化2,2'-二氯二异丙醚（DDE）。使用含3.3 mM甲醇作为共底物的模型水，DDE负载率高达150 µmol l-1 天–1 ，水力停留时间为90％，连续DDE去除效率达到90％以上。 24小时在分批培养中，观察到每微摩尔DDE转化后的化学计量释放2微摩尔氯。从混合培养物中分离出一种菌株，该菌株能够在DDE上生长，作为唯一的能源和碳源，可耐受高达1 mM的DDE浓度。基于16S rRNA测序，该菌株与红球菌属相关。

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《Applied Microbiology and Biotechnology》 |2001年第4期|491-495|共5页
作者
R. Hauck; L. Adrian; P. Wendler; M. Amidjojo; W. Hegemann; H. Görisch;
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Institut für Technischen Umweltschutz Fachgebiet Siedlungswasserwirtschaft Sekr. KF 7 Strasse des 17. Juni 135 10623 Berlin Germany;

Institut für Biotechnologie Fachgebiet Technische Biochemie Sekr. GG1 Seestrasse 13 13353 Berlin Germany;

Institut für Biotechnologie Fachgebiet Technische Biochemie Sekr. GG1 Seestrasse 13 13353 Berlin Germany;

Institut für Technischen Umweltschutz Fachgebiet Siedlungswasserwirtschaft Sekr. KF 7 Strasse des 17. Juni 135 10623 Berlin Germany;

Institut für Technischen Umweltschutz Fachgebiet Siedlungswasserwirtschaft Sekr. KF 7 Strasse des 17. Juni 135 10623 Berlin Germany;

Institut für Biotechnologie Fachgebiet Technische Biochemie Sekr. GG1 Seestrasse 13 13353 Berlin Germany;

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Transformation of 2,2′-dichlorodiisopropyl ether in mixed and pure culture

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