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首页> 外文期刊>Applied Optics >Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells
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Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells

机译：多焦点多光子显微镜的三维时间分辨荧光成像在活细胞中的光敏剂研究

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摘要

Two-photon fluorescence microscopy is widely applied to biology and medicine to study both the structure and dynamic processes in living cells. The main issue is the slow acquisition rate due to the point scanning approach limiting the multimodal detection (x,y,z,t). To extend the performances of this powerful technique, we present a time-resolved multifocal multiphoton microscope (MMM) based on laser amplitude splitting. An array of 8 × 8 foci is created on the sample that gives a direct insight of the fluorescence localization. Four-dimensional (4D) imaging is obtained by combining simultaneous foci scanning, time-gated detection, and z displacement. We illustrate time-resolved MMM capabilities for 4D imaging of a photosensitizer inside living colon cancer cells. The aim of this study is to have a better understanding of the photophysical processes implied in the photosensitizer reactivity.
机译：双光子荧光显微镜已广泛应用于生物学和医学领域，以研究活细胞的结构和动力学过程。主要问题是由于点扫描方法限制了多模式检测（x，y，z，t）而导致的采集速度慢。为了扩展这项强大技术的性能，我们提出了一种基于激光振幅分裂的时间分辨多焦点多光子显微镜（MMM）。在样品上创建了一个8×8焦点的阵列，可以直接了解荧光的定位。通过同时进行焦点扫描，时间选通检测和z位移相结合，获得了四维（4D）成像。我们说明了时间分辨的MMM功能，用于活结肠癌细胞内的光敏剂的4D成像。这项研究的目的是更好地理解光敏剂反应性中隐含的光物理过程。

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