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Self-assembly of cholesterol DNA at liquid crystal/aqueous interface and its application for DNA detection

机译:液晶/水界面胆固醇DNA的自组装及其在DNA检测中的应用

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摘要

In this letter, we report a strategy of detecting the DNA targets by using a thin layer of self-assembled cholesterol-labeled DNA probes at the liquid crystal (LC)/aqueous interface. When the system is exposed to 51 μg/ml of complementary DNA targets, the optical appearance of LC shows a continuous change from dark to bright under the crossed polars within 15 min. No obvious change can be observed when the system is exposed to one or two base-pair mismatch DNA targets. This system provides a principle for label-free and real-time detection of DNA targets without any fluorescent labeling.
机译:在这封信中,我们报告了一种通过在液晶(LC)/水界面使用一层自组装胆固醇标记的DNA探针来检测DNA靶标的策略。当系统暴露于51μg/ ml互补DNA靶标时,LC的光学外观在15分钟内在交叉的极性下显示从暗到亮的连续变化。当系统暴露于一个或两个碱基对错配的DNA靶标时,没有观察到明显的变化。该系统提供了无需任何荧光标记即可无标记,实时检测DNA目标的原理。

著录项

  • 来源
    《Applied Physicsletters》 |2009年第15期|153702.1-153702.3|共3页
  • 作者单位

    Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore;

    Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore;

    Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
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