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Differentiation of Healthy Brain Tissue and Tumors Using Surface-Enhanced Raman Scattering

机译：使用表面增强拉曼散射区分健康的脑组织和肿瘤

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Surface-enhanced Raman scattering (SERS) is a powerful technique for characterization of biological samples. SERS spectra from healthy brain tissue and tumors are obtained by sudden freezing of tissue in liquid nitrogen and crashing and mixing it with a concentrated silver colloidal suspension. The acquired spectra from tissues show significant spectral differences that can be used to identify whether it is from a healthy region or tumor. The most significant change on SERS spectra from the healthy/peripheral brain tissue to tumor is the increase of the ratio of the peaks at around 723 to 655 cm−1. In addition, the spectral changes indicate that the protein content in tumors increases compared to the peripheral/healthy tissue as observed with tumor invasion. The preliminary results show that SERS spectra can be used for a quick diagnosis due to the simplicity of the sample preparation and the speed of the spectral acquisition.
机译：表面增强拉曼散射（SERS）是表征生物样品的强大技术。来自健康大脑组织和肿瘤的SERS光谱是通过将组织在液氮中突然冻结，然后将其与浓缩的银胶体悬浮液混合并使其崩溃而获得的。从组织获得的光谱显示出明显的光谱差异，可用于识别它是来自健康区域还是肿瘤。从健康/周围脑组织到肿瘤的SERS光谱上最显着的变化是在723 cm至655 cm -1 附近的峰比增加。此外，光谱变化表明，与肿瘤侵袭相比，肿瘤的蛋白质含量与周围/健康组织相比有所增加。初步结果表明，由于样品制备的简单性和光谱采集的速度，SERS光谱可用于快速诊断。

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    《Applied Spectroscopy》 |2009年第10期|1095-1100|共6页
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