• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Spectroscopy >Fluorescence Spectroscopy for Rapid Detection and Classification of Bacterial Pathogens
【24h】

Fluorescence Spectroscopy for Rapid Detection and Classification of Bacterial Pathogens

机译：荧光光谱用于细菌病原体的快速检测和分类

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study deals with the rapid detection and differentiation of Escherichia coli, Salmonella, and Campylobacter, which are the most commonly identified commensal and pathogenic bacteria in foods, using fluorescence spectroscopy and multivariate analysis. Each bacterial sample cultured under controlled conditions was diluted in physiologic saline for analysis. Fluorescence spectra were collected over a range of 200–700 nm with 0.5 nm intervals on the PerkinElmer Fluorescence Spectrometer. The synchronous scan technique was employed to find the optimum excitation (λex) and emission (λem) wavelengths for individual bacteria with the wavelength interval (Δλ) being varied from 10 to 200 nm. The synchronous spectra and two-dimensional plots showed two maximum λex values at 225 nm and 280 nm and one maximum λem at 335–345 nm (λem = λex + Δλ), which correspond to the λex = 225 nm, Δλ = 110–120 nm, and λex = 280 nm, Δλ = 60–65 nm. For all three bacterial genera, the same synchronous scan results were obtained. The emission spectra from the three bacteria groups were very similar, creating difficulty in classification. However, the application of principal component analysis (PCA) to the fluorescence spectra resulted in successful classification of the bacteria by their genus as well as determining their concentration. The detection limit was approximately 103–104 cells/mL for each bacterial sample. These results demonstrated that fluorescence spectroscopy, when coupled with PCA processing, has the potential to detect and to classify bacterial pathogens in liquids. The methology is rapid (>10 min), inexpensive, and requires minimal sample preparation compared to standard analytical methods for bacterial detection.
机译：这项研究使用荧光光谱法和多变量分析法，对食品中最常见的共生和致病细菌-大肠杆菌，沙门氏菌和弯曲杆菌进行了快速检测和区分。将在受控条件下培养的每种细菌样品稀释在生理盐水中进行分析。在PerkinElmer荧光光谱仪上以200 nm至700 nm的范围以0.5 nm的间隔收集荧光光谱。同步扫描技术被用于寻找单个细菌的最佳激发波长（λ ex ）和发射波长（λ em ），波长间隔（Δλ）从10开始变化到200 nm同步光谱和二维图显示了在225 nm和280 nm处的两个最大λ ex 值和在335-345 nm处的一个最大λ em （λ em =λ ex +Δλ），分别对应于λ ex = 225 nm，Δλ= 110–120 nm和λ ex = 280 nm，Δλ= 60–65 nm。对于所有三个细菌属，获得了相同的同步扫描结果。三个细菌组的发射光谱非常相似，难以分类。但是，将主成分分析（PCA）应用于荧光光谱可以成功地根据细菌的属对细菌进行分类，并确定其浓度。每个细菌样品的检出限约为10 3 –10 4 细胞/ mL。这些结果表明，荧光光谱结合PCA处理后，有可能检测和分类液体中的细菌病原体。与用于细菌检测的标准分析方法相比，该方法学快速（> 10分钟），价格便宜并且需要最少的样品制备。

著录项

  • 来源
    《Applied Spectroscopy》 |2009年第11期|1251-1255|共5页
  • 作者

  • 作者单位
  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号