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首页> 外文期刊>Applied Surface Science >In vitro cytocompatibility of chitosan/PVA/methylcellulose - Nanocellulose nanocomposites scaffolds using L929 fibroblast cells
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In vitro cytocompatibility of chitosan/PVA/methylcellulose - Nanocellulose nanocomposites scaffolds using L929 fibroblast cells

机译：壳聚糖/ PVA /甲基纤维素的体外细胞相容性-使用L929成纤维细胞的纳米纤维素纳米复合材料支架

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摘要

The present work reports low cost, green synthesis of Nanocellulose (NC) nanoparticles. The biosynthesized nanoparticles were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (ATR-FTIR). The synthesized NC nanoparticles were pure, predominantly spherical in shape with size ranging from 25 nm. The biosynthesized NC nanoparticles have been used for antibacterial and in vitro applications. The antibacterial activity of the prepared CS/PVA/MC-NC1,CS/PVA/MC-NC2, and CS/PVA/MC-NC3 nanocomposites samples was tested against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) due to disk diffusion method. When adding the NC nanoparticles both bacteria were very good inhibition zone was obtained. The transmission NC micrographs depicts spherical with uniform shape and good crystallinity and are composed of nanoparticles with a diameter less than 25 nm the average cube size was 100 nm. The AFM thickness of the CS/PVA/MC-NC1 scaffold was, estimated from the AFM image, was about 10-20 nm and a roughness-like structure was observed. The FESEM film exhibits a scaffold exhibited porous structures. The excellent cell viability of the composite scaffolds was attributed to the good biocompatibility of the CS/PVA/MC-NC3 as well as green fabrication process of the scaffolds. MTT analysis exposed that the samples did not have any toxicity. Since these positive points, these two kinds of scaffolds show appropriate properties for attachment, proliferation, and tendency to form group from L929 cells. In this work, we have prepared nanocellulose by high pressure homogenization process and the resultant nanocellulose was evaluated as fibers in CS/PVA/MC films. (C) 2017 Elsevier B.V. All rights reserved.
机译：本工作报道了纳米纤维素（NC）纳米粒子的低成本，绿色合成。通过透射电子显微镜（TEM），X射线衍射（XRD）和傅里叶变换红外光谱（ATR-FTIR）对生物合成的纳米颗粒进行了表征。合成的NC纳米粒子是纯净的，主要为球形，尺寸范围为25 nm。生物合成的NC纳米颗粒已用于抗菌和体外应用。测试了制备的CS / PVA / MC-NC1，CS / PVA / MC-NC2和CS / PVA / MC-NC3纳米复合材料样品对金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）的抗菌活性。由于磁盘扩散法。当添加NC纳米颗粒时，两种细菌均具有非常好的抑制区。透射NC显微照片描绘了具有均匀形状和良好结晶度的球形，并且由直径小于25 nm的纳米颗粒组成，平均立方体尺寸为100 nm。根据AFM图像估计，CS / PVA / MC-NC1支架的AFM厚度为约10-20nm，并且观察到类似粗糙的结构。 FESEM膜的支架表现出多孔结构。复合支架的优异细胞生存能力归因于CS / PVA / MC-NC3的良好生物相容性以及支架的绿色制造工艺。 MTT分析表明样品没有任何毒性。由于这些积极点，这两种支架显示出适当的附着，增殖和从L929细胞形成群的倾向。在这项工作中，我们通过高压均质工艺制备了纳米纤维素，并将所得纳米纤维素作为CS / PVA / MC薄膜中的纤维进行了评估。 （C）2017 Elsevier B.V.保留所有权利。

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