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首页> 外文期刊>Applied Surface Science >Interaction of phosphorylcholine with fibronectin coatings: Surface characterization and biological performances
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Interaction of phosphorylcholine with fibronectin coatings: Surface characterization and biological performances

机译：磷酸胆碱与纤连蛋白涂层的相互作用：表面表征和生物学性能

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摘要

Coating medical devices with several bioactive molecules is an interesting approach to achieve specific biological targets upon the interaction of the biomaterial with the living environment. In this work, a fluorocarbon polymer (CFx) was first deposited by plasma treatment on stainless steel (SS) substrate and thereafter, coatings containing fibronectin (FN) and phosphorylcholine (PRC) were created for cardiovascular applications. These two biomolecules were chosen to promote endothelialization and to avoid thrombus formation, respectively. Adsorption and grafting techniques were applied - and combined - to accomplish 4 different coatings containing both molecules. However, big challenge was found to characterize a small molecule (PRC: 184 g/mol) interacting with a protein (FN: 450 kD). For the first time XPS, dynamic water contact angle, immunostaining and ToF-SIMS (imaging and depth profiling) analyses were combined to accomplish the characterization of such a coating. The most encouraging biological performances were obtained for samples where FN was grafted to the CFx film followed by the adsorption of PRC: proliferation of endothelial cells and hemocompatibility properties were observed. Promising coatings for cardiovascular applications were developed. The relevance of characterizing the coatings with high sensitive techniques and the further correlation with their biological performances were evidenced. (C) 2016 Elsevier B.V. All rights reserved.
机译：在生物材料与生活环境相互作用后，用几种生物活性分子包被医疗器械是一种实现特定生物学目标的有趣方法。在这项工作中，首先通过等离子体处理将碳氟化合物聚合物（CFx）沉积在不锈钢（SS）基底上，然后创建包含纤连蛋白（FN）和磷酰胆碱（PRC）的涂层，用于心血管应用。选择这两种生物分子分别促进内皮化和避免血栓形成。应用和结合了吸附和接枝技术，以完成包含两种分子的4种不同涂层。然而，发现挑战在于表征与蛋白质（FN：450 kD）相互作用的小分子（PRC：184 g / mol）。 XPS首次将动态水接触角，免疫染色和ToF-SIMS（成像和深度分析）分析相结合，以完成这种涂层的表征。对于将FN接枝到CFx膜上然后再吸附PRC的样品，获得了最令人鼓舞的生物学性能：观察到内皮细胞的增殖和血液相容性。开发了用于心血管应用的有前途的涂料。证明了用高灵敏度技术表征涂层的相关性及其与生物学性能的进一步相关性。 （C）2016 Elsevier B.V.保留所有权利。

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  • 来源
    《Applied Surface Science》 |2017年第2期|1613-1622|共10页
  • 作者

    Montano-Machado Vanessa; Noel Celine; Chevallier Pascale; Turgeon Stephane; Houssiau Laurent; Pauthe Emmanuel; Pireaux Jean-Jacques; Mantovani Diego;

  • 作者单位

    Univ Laval, Lab Biomat & Bioengn, Dept Min Met Mat Engn, Univ Campus,PLT 1745G, Quebec City, PQ G1V 0A6, Canada|Univ Laval, Univ Hosp Res Ctr, Univ Campus,PLT 1745G, Quebec City, PQ G1V 0A6, Canada|Univ Cergy Pontoise, ERRMECe, Site St Martin,2 Ave Adolphe Chauvin, F-95302 Cergy Pontoise, France;

    Univ Namur, Res Ctr Phys Matter & Radiat PMR, 61 Rue Bruxelles, B-5000 Namur, Belgium;

    Univ Laval, Lab Biomat & Bioengn, Dept Min Met Mat Engn, Univ Campus,PLT 1745G, Quebec City, PQ G1V 0A6, Canada|Univ Laval, Univ Hosp Res Ctr, Univ Campus,PLT 1745G, Quebec City, PQ G1V 0A6, Canada;

    Univ Laval, Lab Biomat & Bioengn, Dept Min Met Mat Engn, Univ Campus,PLT 1745G, Quebec City, PQ G1V 0A6, Canada|Univ Laval, Univ Hosp Res Ctr, Univ Campus,PLT 1745G, Quebec City, PQ G1V 0A6, Canada;

    Univ Namur, Res Ctr Phys Matter & Radiat PMR, 61 Rue Bruxelles, B-5000 Namur, Belgium;

    Univ Cergy Pontoise, ERRMECe, Site St Martin,2 Ave Adolphe Chauvin, F-95302 Cergy Pontoise, France;

    Univ Namur, Res Ctr Phys Matter & Radiat PMR, 61 Rue Bruxelles, B-5000 Namur, Belgium;

    Univ Laval, Lab Biomat & Bioengn, Dept Min Met Mat Engn, Univ Campus,PLT 1745G, Quebec City, PQ G1V 0A6, Canada|Univ Laval, Univ Hosp Res Ctr, Univ Campus,PLT 1745G, Quebec City, PQ G1V 0A6, Canada;

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  • 正文语种 eng
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  • 关键词

    Fibronectin; Phosphorylcholine; Grafting; Endothelialization; Hemocompatibility; Biomaterials;

    机译：纤连蛋白;磷酸胆碱;接枝;内皮化;血液相容性;生物材料;

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