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首页> 外文期刊>Applied Surface Science >Effect of surface structure and wettability of DLC and N-DLC thin films on adsorption of glycine
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Effect of surface structure and wettability of DLC and N-DLC thin films on adsorption of glycine

机译：DLC和N-DLC薄膜的表面结构和润湿性对甘氨酸吸附的影响

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Diamond-like carbon (DLC) is known to have excellent biocompatibility. Various samples of DLC and nitrogen-doped DLC thin films (N-DLC) were deposited onto silicon substrates using plasma-enhanced chemical vapour deposition (PECVD). Subsequently, the adsorption of amino acid glycine onto the surfaces of the thin films was investigated to elucidate the mechanisms involved in protein adhesion. The physicochemical characteristics of the surfaces, before and after adsorption of glycine, were investigated using Fourier transfer infrared (FTIR), Raman spectroscopy, spectroscopic ellipsometry (SE) and contact angle (0). The Raman study highlighted decrease slightly in the ID/1G ratio at low levels of N (5.4at.%), whilst increasing the nitrogen dopant level (>5.4at.%) resulted in a increase of the ID/IG ratio, and the FTIR band at related to C=N. Following exposure to glycine solutions, the presence of Raman bands at 1727 cm~(-1) and 1200 cm~(-1), and FTIR bands at 1735 cm1 indicates that the adsorption of glycine onto the surfaces has taken place. These results which obtained from SE and surface free energy, show that low levels of nitrogen doping in DLC enhances the adsorption of the amino acid, while, increased doping led to a reduced adsorption, as compared to undoped DLC. Glycine is bound to the surface of the DLC films via both de-protonated carboxyl and protonated amino groups while, in the case of N-DLC gylcine was bound to the surface via anionic carboxyl groups and the amino group did not interact strongly with the surface. Doping of DLC may allow control of protein adsorption to the surface.
机译：类金刚石碳（DLC）具有出色的生物相容性。使用等离子增强化学气相沉积（PECVD）将各种DLC和氮掺杂DLC薄膜（N-DLC）样品沉积到硅基板上。随后，研究了氨基酸甘氨酸在薄膜表面的吸附，以阐明参与蛋白质粘附的机制。使用傅里叶转移红外（FTIR），拉曼光谱，椭圆偏振光谱（SE）和接触角（0）研究了甘氨酸吸附前后表面的理化特性。拉曼研究强调在低氮水平（5.4at。％）时ID / 1G比例略有​​降低，而氮掺杂水平（> 5.4at。％）的升高导致ID / IG比例的提高，并且与C ＝ N有关的FTIR频带。暴露于甘氨酸溶液后，在1727 cm-1（-1）和1200 cm-1（-1）处的拉曼谱带和在1735 cm1处的FTIR谱带的存在表明甘氨酸已吸附在表面上。从SE和表面自由能获得的这些结果表明，与未掺杂的DLC相比，DLC中低水平的氮掺杂会增强氨基酸的吸附，而增加的掺杂会导致吸附降低。甘氨酸通过去质子化的羧基和质子化的氨基结合到DLC膜的表面，而在N-DLC情况下，甘氨酸通过阴离子的羧基结合到表面，并且氨基不与表面强烈相互作用。掺杂DLC可以控制蛋白质吸附到表面上。

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