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首页> 外文期刊>Applied Surface Science >Immobilization of glucose oxidase using CoFe_2O_4/SiO_2 nanoparticles as carrier
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Immobilization of glucose oxidase using CoFe_2O_4/SiO_2 nanoparticles as carrier

机译：以CoFe_2O_4 / SiO_2纳米粒子为载体固定葡萄糖氧化酶

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摘要

Aminated-CoFe_2O_4/SiO_2 magnetic nanoparticles (NPs) were prepared from primary silica particles using modified StOber method. Glucose oxidase (GOD) was immobilized on CoFe_2O_4/SiO_2 NPs via cross-linking with glutaraldehyde (GA). The optimal immobilization condition was achieved with 1% (v/v) GA, cross-linking time of 3 h, solution pH of 7.0 and 0.4 mg GOD (in 3.0 mg carrier). The immobilized GOD showed maximal catalytic activity at pH 6.5 and 40℃. After immobilization, the GOD exhibited improved thermal, storage and operation stability. The immobilized GOD still maintained 80% of its initial activity after the incubation at 50℃ for 25 min, whereas free enzyme had only 20% of initial activity after the same incubation. After kept at 4℃ for 28 days, the immobilized and free enzyme retained 87% and 40% of initial activity, respectively. The immobilized GOD maintained approximately 57% of initial activity after reused 7 times. The K_M (Michaelis-Menten constant) values for immobilized GOD and free GOD were 14.6 raM and 27.1 mM, respectively.
机译：使用改良的StOber方法从一级二氧化硅颗粒制备了胺化的CoFe_2O_4 / SiO_2磁性纳米颗粒（NPs）。通过与戊二醛（GA）交联将葡萄糖氧化酶（GOD）固定在CoFe_2O_4 / SiO_2 NP上。最佳固定条件是通过1％（v / v）的GA，3小时的交联时间，7.0的溶液pH和0.4 mg的GOD（在3.0 mg的载体中）实现的。固定化的GOD在pH 6.5和40℃下表现出最大的催化活性。固定后，GOD表现出改善的热，储存和操作稳定性。固定化的GOD在50℃孵育25分钟后仍保持其初始活性的80％，而游离酶在相同孵育后仅具有初始活性的20％。在4℃下放置28天后，固定化酶和游离酶分别保留了初始活性的87％和40％。重复使用7次后，固定的GOD保持初始活性的约57％。固定GOD和游离GOD的K_M（Michaelis-Menten常数）值分别为14.6 raM和27.1 mM。

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  • 来源
    《Applied Surface Science》 |2011年第13期|p.5739-5745|共7页
  • 作者

    Hai Wang; JunHuang; Chao Wang; DapengLi; LiyunDing; YunHan;

  • 作者单位

    Key Laboratory of Fiber Optic Sensing Technology and Information Processing of Ministry of Education, Wuhan University of Technology, Wuhan 430070, China;

    Key Laboratory of Fiber Optic Sensing Technology and Information Processing of Ministry of Education, Wuhan University of Technology, Wuhan 430070, China;

    Key Laboratory of Fiber Optic Sensing Technology and Information Processing of Ministry of Education, Wuhan University of Technology, Wuhan 430070, China;

    Key Laboratory of Fiber Optic Sensing Technology and Information Processing of Ministry of Education, Wuhan University of Technology, Wuhan 430070, China;

    Key Laboratory of Fiber Optic Sensing Technology and Information Processing of Ministry of Education, Wuhan University of Technology, Wuhan 430070, China;

    Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, Castle Point on Hudson, Hoboken, NJ 07030, USA;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    CoFe_2O_4/SiO_2; Nanoparticles; Immobilization; Glucose oxidase;

    机译：CoFe_2O_4 / SiO_2;纳米颗粒;固定化;葡萄糖氧化酶;

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