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Conducting Polymer Film-Based Immunosensors Using Carbon Nanotube/Antibodies Doped Polypyrrole

机译:使用碳纳米管/抗体掺杂的聚吡咯传导基于聚合物膜的免疫传感器

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Carbon nanotube/polypyrrole/antibodies polymer films were synthesized successfully on microelec-trodes by electrochemical deposition. Electropolymerization was performed at optimal range between -0.8 and +0.8 V at a scan rate of 50 mV s~(-1) in an electrochemical mini-cell containing monomer pyrroles, carbon nanotubes, and goat IgGs. The conducting polymer films were characterized by Fourier transform infrared spectrometry, Raman spectra, and Field emission scanning electron microscopy. And then, it was prepared for immunosensor application to determine anti-goat IgGs. The results show that a linear range between 0.05 and 0.7 u,g ml"1 for anti-goat IgGs detection was observed for immunosensor, a detection limit as low as 0.05 (o-gml~(-1) and a response time of 1 min. The effect parameters of electropolymerization process on immunosensor response are also studied. It found that the immunosensor well active in 1.5 mg ml~(-1) CNT concentration, 2.5 mM pyrrole, 10 u-g ml~(-1) goat IgGs.
机译:通过电化学沉积在微电极上成功合成了碳纳米管/聚吡咯/抗体聚合物薄膜。在包含单体吡咯,碳纳米管和山羊IgG的电化学微型电池中,以50 mV s〜(-1)的扫描速率在-0.8至+0.8 V的最佳范围内进行电聚合。通过傅立叶变换红外光谱,拉曼光谱和场发射扫描电子显微镜对导电聚合物薄膜进行表征。然后,准备将其用于免疫传感器以确定抗山羊IgG。结果表明,免疫传感器的抗山羊IgGs检测线性范围在0.05至0.7 u,g ml-1之间,检测限低至0.05(o-gml〜(-1),响应时间为1研究了电聚合过程对免疫传感器响应的影响参数,发现该免疫传感器在1.5 mg ml〜(-1)CNT浓度,2.5 mM吡咯,10 ug ml〜(-1)山羊IgGs中具有良好的活性。

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