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首页> 外文期刊>Applied Surface Science >Bilayer porous scaffold based on poly-(ε-caprolactone) nanofibrous membrane and gelatin sponge for favoring cell proliferation
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Bilayer porous scaffold based on poly-(ε-caprolactone) nanofibrous membrane and gelatin sponge for favoring cell proliferation

机译：基于聚（ε-己内酯）纳米纤维膜和明胶海绵的双层多孔支架，有利于细胞增殖

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摘要

Electrospun poly-(ε-caprolactone) (PCL) nanofibers has been widely used in the medical prosthesis. However, poor hydrophilicity and the lack of natural recognition sites for covalent cell-recognition signal molecules to promote cell attachment have limited its utility as tissue scaffolds. In this study, Bilayer porous scaffolds based on PCL electrospun membranes and gelatin (GE) sponges were fabricated through soft hydrolysis of PCL electrospun followed by grafting gelatin onto the fiber surface, through crosslinking and freeze drying treatment of additional gelatin coat and grafted gelatin surface. GE sponges were stably anchored on PCL membrane surface with the aid of grafted GE molecules. The morphologies of bilayer porous scaffolds were observed through SEM. The contact angle of the scaffolds was 0°, the mechanical properties of scaffolds were measured by tensile test, Young's moduli of PCL scaffolds before and after hydrolysis are 66-77.3 MPa and 62.3-75.4 MPa, respectively. Thus, the bilayer porous scaffolds showed excellent hydrophilic surface and desirable mechanical strength due to the soft hydrolysis and GE coat. The cell culture results showed that the adipose derived mesenchymal stem cells did more favor to adhere and grow on the bilayer porous scaffolds than on PCL electrospun membranes. The better cell affinity of the final bilayer scaffolds not only attributed to the surface chemistry but also the introduction of bilayer porous structure.
机译：电纺聚（ε-己内酯）（PCL）纳米纤维已广泛用于医疗修复体中。然而，较差的亲水性和共价细胞识别信号分子缺乏天然识别位点以促进细胞附着限制了其作为组织支架的用途。在这项研究中，基于PCL电纺膜和明胶（GE）海绵的双层多孔支架是通过PCL电纺的软水解，然后将明胶接枝到纤维表面上，通过交联和冷冻干燥额外的明胶涂层和接枝的明胶表面而制成的。借助接枝的GE分子，将GE海绵稳定地锚固在PCL膜表面上。通过SEM观察双层多孔支架的形态。支架的接触角为0°，通过拉伸试验测量支架的力学性能，水解前后的PCL支架的杨氏模量分别为66-77.3 MPa和62.3-75.4 MPa。因此，双层多孔支架由于柔软的水解和GE涂层而显示出优异的亲水性表面和所需的机械强度。细胞培养结果表明，与PCL电纺膜相比，脂肪来源的间充质干细胞在双层多孔支架上更易于粘附和生长。最终双层支架的更好的细胞亲和力不仅归因于表面化学，而且归因于双层多孔结构的引入。

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  • 来源
    《Applied Surface Science》 |2011年第5期|p.1670-1676|共7页
  • 作者

    Zhihua Zhou; Yang Zhou; Yiwang Chen; Huarong Nie; Yang Wang; Fan Li; Yan Zheng;

  • 作者单位

    Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China;

    Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China;

    Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China,Institute of Polymers, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China;

    Institute of Polymers, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China;

    First Affiliated Hospital, Nanchang University, 17 Yongwaizheng Road, Nanchang 330006, China;

    Institute of Polymers, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China;

    Institute of Polymers, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China;

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  • 正文语种 eng
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  • 关键词

    bilayer porous scaffold; poly-(ε-caprolactone) nanofibrous; membrane; gelatin sponge;

    机译：双层多孔支架聚（ε-己内酯）纳米纤维;膜;明胶海绵;

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