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Albumin adsorption on oxide thin films studied by spectroscopic ellipsometry

机译：椭圆偏振光谱法研究白蛋白在氧化物薄膜上的吸附

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摘要

Thin films of tantalum, niobium, zirconium and titanium oxides were deposited by reactive magnetron sputtering and their wettability and surface energy, optical properties, roughness, chemical composition and microstructure were characterized using contact angle measurements, spectroscopic ellipsometry, profilometry, X-ray photoelectron spectroscopy and X-ray diffraction, respectively. The purpose of the work was to correlate the surface properties of the films to the Bovine Serum Albumin (BSA) adsorption, as a first step into the development of an initial in vitro test of the films biocompatibility, based on standardized protein adsorption essays. The films were immersed into BSA solutions with different protein concentrations and protein adsorption was monitored in situ by dynamic ellipsometry; the adsorption-rate was dependent on the solution concentration and the immersion time. The overall BSA adsorption was studied in situ using spectroscopic ellipsometry and it was found to be influenced by the wettability of the films; larger BSA adsorption occurred on the more hydrophobic surface, the ZrO_2 film. On the Ta_2O_5, Nb_2O_5 and TiO_2 films, hydrophilic surfaces, the overall BSA adsorption increased with the surface roughness or the polar component of the surface energy.
机译：通过反应磁控溅射沉积钽，铌，锆和钛氧化物的薄膜，并通过接触角测量，椭圆偏振光谱，轮廓分析，X射线光电子能谱表征了它们的润湿性和表面能，光学性质，粗糙度，化学成分和微观结构。和X射线衍射。这项工作的目的是将薄膜的表面性质与牛血清白蛋白（BSA）吸附相关联，这是基于标准化蛋白质吸附论文开发薄膜生物相容性的体外试验的第一步。将膜浸入具有不同蛋白质浓度的BSA溶液中，并通过动态椭偏仪原位监测蛋白质的吸附。吸附速率取决于溶液浓度和浸泡时间。使用椭偏光谱法对BSA的整体吸附进行了原位研究，发现其受膜的润湿性的影响。较大的BSA吸附发生在疏水性更高的表面ZrO_2膜上。在Ta_2O_5，Nb_2O_5和TiO_2薄膜的亲水表面上，总BSA吸附随表面粗糙度或表面能的极性成分而增加。

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  • 来源
    《Applied Surface Science》 |2011年第5期|p.1711-1718|共8页
  • 作者

    P. Silva-Bermudez; S.E. Rodil; S. Muhl;

  • 作者单位

    Institute: de lnvestigaciones en Materiales, Universidad National Autonoma de Mexico, Circuito Exterior s, C.U., 04510, Mexico D.F., Mexico,Unidad de Posgrado, Facultad de Odontologia, Universidad National Autonoma de Mexico, CU, 04510, Mexico D.F., Mexico;

    Institute: de lnvestigaciones en Materiales, Universidad National Autonoma de Mexico, Circuito Exterior s, C.U., 04510, Mexico D.F., Mexico;

    Institute: de lnvestigaciones en Materiales, Universidad National Autonoma de Mexico, Circuito Exterior s, C.U., 04510, Mexico D.F., Mexico;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    protein adsorption; ellipsometry; metal oxide thin film; albumin;

    机译：蛋白质吸附;椭圆仪金属氧化物薄膜;白蛋白;

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