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Cystamine immobilization on TiO_2 film surfaces and the influence on inhibition of collagen-induced platelet activation

机译：胱胺固定在TiO_2薄膜表面上及其对抑制胶原诱导的血小板活化的影响

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摘要

Poor haemocompatibility is a main issue of artificial cardiovascular materials in clinical application. Nitric oxide (NO), produced by vascular endothelial cells, is a well known inhibitor of platelet adhesion and activation. Thus, NO-releasing biomaterials are beneficial for improving haemocompatibility of blood-contacting biomedical devices. In this paper, a novel method was developed for enhancement of haemocompatibility by exploiting endogenous NO donors. TiO_2 films were firstly synthesized on Si (100) wafers via unbalanced magnetron sputtering technology, and then polydopamine was grafted on TiO_2 films and used as a linker for further immobilization of cystamine. The obtained surfaces were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analysis. NO generation is evaluated by saville-griess reagents, and it shows that cystamine immobilized samples are able to catalytically generate NO by decomposing endogenous S-nitrosothiols (RSNO). In vitro platelet adhesion results reveal that cystamine modified surfaces can inhibit collagen-induced platelet activation. ELISA analysis reveals that cGMP in platelets obviously increases on cystamine immobilized surface, which suggests the reducing of platelet activation is through NO/cGMP signal channel. It can be concluded that cystamine immobilized surface shows better blood compatibility by catalyzing NO release from the endogenous NO donor. It may be a promising method for improvement of haemocompatibility of blood-contacting implants.
机译：血液相容性差是临床上人造心血管材料的主要问题。血管内皮细胞产生的一氧化氮（NO）是众所周知的血小板粘附和活化抑制剂。因此，释放NO的生物材料有益于改善血液接触生物医学装置的血液相容性。在本文中，开发了一种通过利用内源性NO供体来增强血液相容性的新方法。首先通过不平衡磁控溅射技术在Si（100）晶片上合成TiO_2薄膜，然后将聚多巴胺接枝到TiO_2薄膜上，并用作进一步固定胱胺的连接剂。通过扫描电子显微镜（SEM），傅立叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）分析来表征获得的表面。用saville-griess试剂评估NO的产生，结果表明固定有胱胺的样品能够通过分解内源性S-亚硝基硫醇（RSNO）催化产生NO。体外血小板粘附结果显示，胱胺修饰的表面可以抑制胶原蛋白诱导的血小板活化。 ELISA分析表明，在固定有胱胺的表面上血小板中的cGMP明显增加，这表明血小板活化的减少是通过NO / cGMP信号通道。可以得出结论，固定有胱胺的表面通过催化内源性NO供体释放NO表现出更好的血液相容性。它可能是改善血液接触植入物血液相容性的一种有前途的方法。

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  • 来源
    《Applied Surface Science》 |2011年第5期|p.1776-1783|共8页
  • 作者

    Yujuan Zhou; Yajun Weng; Liping Zhang; Fengjuan Jing; Nan Huang; Junying Chen;

  • 作者单位

    Key Lab. of Advanced Technology for Materials of Chinese Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;

    Key Lab. of Advanced Technology for Materials of Chinese Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;

    Key Lab. of Advanced Technology for Materials of Chinese Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;

    Key Lab. of Advanced Technology for Materials of Chinese Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;

    Key Lab. of Advanced Technology for Materials of Chinese Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;

    Key Lab. of Advanced Technology for Materials of Chinese Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;

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  • 正文语种 eng
  • 中图分类
  • 关键词

    cystamine; nitric oxide; catalytic generation; cyclic guanosine monophosphate; collagen-induced platelet activation;

    机译：胱胺一氧化氮;催化生成;环状鸟苷一磷酸;胶原诱导的血小板活化;

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