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首页> 外文期刊>Applied Surface Science >Immobilization of cholesterol oxidase to finely dispersed silica-coated maghemite nanoparticles based magnetic fluid
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Immobilization of cholesterol oxidase to finely dispersed silica-coated maghemite nanoparticles based magnetic fluid

机译：将胆固醇氧化酶固定在精细分散的二氧化硅涂层磁赤铁矿纳米颗粒基磁性流体上

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摘要

In the recent years, the potential applicability of magnetic nanoparticles (MNPs) has witnessed a significant increase in interest towards the medical field, in particular, towards the usage of novel nanoparticles in diagnostics and disease treatment, respectively. In a present study, cholesterol oxidase (ChOx) was covalently immobilized to magnetic nanoparticles of maghemite (γ-Fe_2O_3) and fuerther functionalized by silica (SiO_2) and amino-silane molecules. The activity of the bound enzyme was retained up to 60%, respectively. The binding of cholesterol oxidase was confirmed using FT-IR spectrophotometer. SEM analysis showed uniformly dispersed functional magnetic nanoparticles, which ranged in size from 22.5 to 50.8 nm, surrounded by amorphous silica. In this paper, the potential applications of chemically modified magnetic nanoparticles as carriers for cholesterol oxidase and other enzymes are discussed.
机译：近年来，磁性纳米颗粒（MNP）的潜在适用性已显着提高了对医学领域的兴趣，尤其是对分别在诊断和疾病治疗中使用新型纳米颗粒的兴趣。在本研究中，胆固醇氧化酶（ChOx）被共价固定在磁赤铁矿（γ-Fe_2O_3）的磁性纳米粒子上，并进一步被二氧化硅（SiO_2）和氨基硅烷分子官能化。结合的酶的活性分别保持高达60％。使用FT-IR分光光度计确认胆固醇氧化酶的结合。 SEM分析显示均匀分散的功能性磁性纳米颗粒，其尺寸为22.5至50.8nm，被无定形二氧化硅包围。在本文中，讨论了化学修饰的磁性纳米粒子作为胆固醇氧化酶和其他酶的载体的潜在应用。

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  • 来源
    《Applied Surface Science》 |2010年第14期|p.4596-4600|共5页
  • 作者

    Franja Sulek; Zeljko Knez; Maja Habulin;

  • 作者单位

    University of Maribor, Faculty of Chemistry and Chemical Engineering, Laboratory for Separation Processes and Product Design, Smetanova17, SI-2000 Maribor, Slovenia;

    rnUniversity of Maribor, Faculty of Chemistry and Chemical Engineering, Laboratory for Separation Processes and Product Design, Smetanova17, SI-2000 Maribor, Slovenia;

    rnUniversity of Maribor, Faculty of Chemistry and Chemical Engineering, Laboratory for Separation Processes and Product Design, Smetanova17, SI-2000 Maribor, Slovenia;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    magnetic nanoparticles; magnetic carrier; enzyme immobilization; cholesterol oxidase;

    机译：磁性纳米粒子磁性载体酶固定化胆固醇氧化酶;

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