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DNA immobilization on polymer-modified Si surface by controlling pH

机译:通过控制pH将DNA固定在聚合物修饰的Si表面上

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摘要

A novel approach based on polymer-modified Si surface as DNA sensor platforms is presented. The polymer-modified Si surface was prepared by using 3-(methacryloxypropyl)trimethoxysilane [7-MPS] and poly(acrylamide) [PAAm]. Firstly, a layer of -γ-MPS was formed on the hydroxylated silicon surface as a monolayer and then modified with different molecular weight of PAAm to form polymer-modified surface. The polymer-modified Si surface was used for dsDNA immobilization. All steps about formation of layer structure were characterized by ellipsometry, atomic force microscopy (AFM), attenuated total reflectance Fourier transformed infrared (ATR-FTIR), and contact angle (CA) measurements. We found that in this case the amount of dsDNA immobilized onto the surface was dictated by the electrostatic interaction between the substrate surface and the DNA Our results thus demonstrated that DNA molecules could be immobilized differently onto the polymer-modified support surface via electrostatic interactions.
机译:提出了一种基于聚合物修饰的硅表面作为DNA传感器平台的新颖方法。通过使用3-(甲基丙烯酰氧基丙基)三甲氧基硅烷[7-MPS]和聚(丙烯酰胺)[PAAm]来制备聚合物改性的Si表面。首先,在羟基化硅表面上以单层形式形成-γ-MPS层,然后用不同分子量的PAAm进行改性以形成聚合物改性的表面。聚合物修饰的Si表面用于dsDNA固定。关于层结构形成的所有步骤均通过椭圆光度法,原子力显微镜(AFM),衰减全反射傅立叶变换红外光谱(ATR-FTIR)和接触角(CA)进行了表征。我们发现,在这种情况下,固定在表面上的dsDNA的量取决于底物表面与DNA之间的静电相互作用。我们的结果因此证明,DNA分子可以通过静电相互作用以不同的方式固定在聚合物修饰的支持物表面上。

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