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首页> 外文期刊>Applied Surface Science >Afm Images Of G_1-phase Premature Condensed Chromosomes: Evidence For 30 Nm Changed To 50 Nm Chromatin Fibers
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Afm Images Of G_1-phase Premature Condensed Chromosomes: Evidence For 30 Nm Changed To 50 Nm Chromatin Fibers

机译：G_1相过早浓缩染色体的Afm图像：将30 Nm的证据转变为50 Nm的染色质纤维

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摘要

To gain evidence for 30 nm changed to 50 nm chromatin fibers, we used atomic force microscopy (AFM) to study the ultrastructural organization of G_1-phase premature condensed chromosomes (PCC). The surface of early G|-phase PCC is smooth and fibrous structures exist around the chromatids. The height of early G_1-phase PCC is about 410 nm and the width is 1.07 ± 0.1 1 μm (n = 30). At late G_1-phase, the surface becomes globular. The height of late Gl-phase PCC is about 370 nm and the width is 845.04 ± 82.84 nm (n = 30). Phase image reveals that early G_1-phase PCC is composed of 50 nm (48.91 ± 6.63 nm, n = 30) chromatin fibers and these 50 nm chromatin fibers tangle together, while late G_1 phase PCC is composed of 30 nm (30.96 ± 4.07 nm, n = 30) chromatin fibers. At high magnification, fibers existing around the chromatids become clear in early G_1-phase PCC. Chromatin fibers revealed by closer view of the end of chromatid are about 50 nm. In late G_1-phase PCC, the surface presents globular structures. The shape of these globular structures is regular and the diameter is 118.96 ± 11.70 nm (n = 30). Our results clearly show that 30 nm chromatin fibers change to 50 nm chromatin fibers in G_1-phase PCC and suggest that 50 nm chromatin fibers are the basic component of the mitotic chromosomes.
机译：为了获得从30 nm到50 nm染色质纤维的证据，我们使用原子力显微镜（AFM）研究了G_1相早缩染色体（PCC）的超微结构。早期G相PCC的表面是光滑的，并且在染色单体周围存在纤维结构。早期G_1相PCC的高度约为410 nm，宽度为1.07±0.1 1μm（n = 30）。在G_1后期，表面变成球形。晚期Gl相PCC的高度为约370nm，宽度为845.04±82.84nm（n ＝ 30）。相图显示，早期G_1相PCC由50 nm（48.91±6.63 nm，n = 30）染色质纤维组成，并且这些50 nm染色质纤维缠结在一起，而晚期G_1相PCC由30​​ nm（30.96±4.07 nm）组成，n = 30）染色质纤维。在高倍率下，染色质周围存在的纤维在早期的G_1相PCC中变得清晰。通过观察染色单体末端可见的染色质纤维约为50 nm。在晚期的G_1相PCC中，表面呈球状结构。这些球状结构的形状是规则的，直径为118.96±11.70 nm（n = 30）。我们的结果清楚地表明，在G_1期PCC中，30 nm染色质纤维变为50 nm染色质纤维，这表明50 nm染色质纤维是有丝分裂染色体的基本组成部分。

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