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首页> 外文期刊>Applied Surface Science >Dna Hybridization And Phosphinothricin Acetyltransferase Gene Sequence Detection Based On Zirconiaanogold Film Modified Electrode
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Dna Hybridization And Phosphinothricin Acetyltransferase Gene Sequence Detection Based On Zirconiaanogold Film Modified Electrode

机译：氧化锆/纳米金膜修饰电极的Dna杂交和膦丝菌素乙酰基转移酶基因序列检测

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摘要

This study reports a novel electrochemical DNA biosensor based on zirconia (ZrO_2) and gold nanoparticles (NG) film modified glassy carbon electrode (GCE). NG was electrodeposited onto the glassy carbon electrode at 1.5 V, and then zirconia thin film on the NG/GCE was fabricated by cyclic voltammetric method (CV) in an aqueous electrolyte of ZrOCl_2 and KCl at a scan rate of 20 mV/s. DNA probes were attached onto the ZrO_2/ NG/GCE due to the strong binding of the phosphate group of DNA with the zirconia film and the excellent biocompatibility of nanogold with DNA. CV and electrochemical impedance spectroscopy (EIS) were used to characterize the modification of the electrode and the probe DNA immobilization. The electrochemical response of the DNA hybridization was measured by differential pulse voltammetry (DPV) using methylene blue (MB) as the electroactive indicator. After the hybridization of DNA probe (ssDNA) with the complementary DNA (cDNA), the cathodic peak current of MB decreased obviously. The difference of the cathodic peak currents of MB between before and after the hybridization of the probe DNA was used as the signal for the detection of the target DNA. The sequence-specific DNA of phosphinothricin acetyltransferase (PAT) gene in the transgenic plants was detected with a detection range from 1.0 × 10~(-10) to 1.0 × 10~(-6) mol/L, and a detection limit of 3.1 × 10~(-11) mol/L.
机译：这项研究报告了一种新型的基于氧化锆（ZrO_2）和金纳米粒子（NG）膜修饰玻碳电极（GCE）的电化学DNA生物传感器。在1.5 V下将NG电沉积到玻璃碳电极上，然后在20rVl / s的扫描速度下，通过循环伏安法（CV）在ZrOCl_2和KCl的水性电解质中在NG / GCE上制备氧化锆薄膜。由于DNA的磷酸基团与氧化锆膜的牢固结合以及纳米金与DNA的优异生物相容性，DNA探针被连接到ZrO_2 / NG / GCE上。 CV和电化学阻抗谱（EIS）用于表征电极修饰和探针DNA固定化。 DNA杂交的电化学响应通过使用亚甲基蓝（MB）作为电活性指示剂的差示脉冲伏安法（DPV）进行测量。 DNA探针（ssDNA）与互补DNA（cDNA）杂交后，MB的阴极峰值电流明显降低。探针DNA杂交前后的MB的阴极峰值电流之差用作检测靶DNA的信号。在转基因植物中检测到草丁膦乙酰转移酶（PAT）基因的序列特异性DNA，检测范围为1.0×10〜（-10）至1.0×10〜（-6）mol / L，检测极限为3.1 ×10〜（-11）摩尔/升

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